QTL mapping of web blotch resistance in peanut by high-throughput genome-wide sequencing

被引：23

作者：

Liu, Hua ^{[1
,2
]}

Sun, Ziqi ^{[2
]}

Zhang, Xinyou ^{[2
]}

Qin, Li ^{[2
]}

Qi, Feiyan ^{[2
]}

Wang, Zhenyu ^{[3
]}

Du, Pei ^{[2
]}

Xu, Jing ^{[2
]}

Zhang, Zhongxin ^{[2
]}

Han, Suoyi ^{[2
]}

Li, Shaojian ^{[3
]}

Gao, Meng ^{[3
]}

Zhang, Lina ^{[2
]}

Cheng, Yujie ^{[2
]}

Zheng, Zheng ^{[2
]}

Huang, Bingyan ^{[2
]}

Dong, Wenzhao ^{[2
]}

机构：

[1] Shenyang Agr Univ, Coll Agron, Shenyang 110866, Peoples R China

[2] Henan Acad Agr Sci, Henan Prov Key Lab Genet Improvement Oil Crops, Key Lab Oil Crops Huang Huai Hai Plains, Ind Crops Res Inst,Minist Agr & Rural Affairs, Zhengzhou 450002, Peoples R China

[3] Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China

来源：

BMC PLANT BIOLOGY | 2020年 / 20卷 / 01期

基金：

中国国家自然科学基金;

关键词：

QTL mapping; Peanut; Web blotch resistance; Resequencing; ARACHIDICOLA;

D O I：

10.1186/s12870-020-02455-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

BackgroundWeb blotch is one of the most important foliar diseases worldwide in peanut (Arachis hypogaea L.). The identification of quantitative trait loci (QTLs) for peanut web blotch resistance represents the basis for gene mining and the application of molecular breeding technologies.ResultsIn this study, a peanut recombinant inbred line (RIL) population was used to map QTLs for web blotch resistance based on high-throughput genome-wide sequencing. Frequency distributions of disease grade and disease index in five environments indicated wide phenotypic variations in response to web blotch among RILs. A high-density genetic map was constructed, containing 3634 bin markers distributed on 20 peanut linkage groups (LGs) with an average genetic distance of 0.5cM. In total, eight QTLs were detected for peanut web blotch resistance in at least two environments, explaining from 2.8 to 15.1% of phenotypic variance. Two major QTLs qWBRA04 and qWBRA14 were detected in all five environments and were linked to 40 candidate genes encoding nucleotide-binding site leucine-rich repeat (NBS-LRR) or other proteins related to disease resistances.ConclusionsThe results of this study provide a basis for breeding peanut cultivars with web blotch resistance.

引用

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