Genome-wide Identification and Characterization of Heat Shock Protein Family Reveals Role in Development and Stress Conditions in Triticum aestivum L.

被引:56
作者
Kumar, Ashish [1 ]
Sharma, Saloni [2 ]
Chunduri, Venkatesh [2 ]
Kaur, Amandeep [2 ]
Kaur, Satinder [3 ]
Malhotra, Nikhil [2 ]
Kumar, Aman [2 ]
Kapoor, Payal [2 ]
Kumari, Anita [2 ]
Kaur, Jaspreet [4 ]
Sonah, Humira [2 ]
Garg, Monika [2 ]
机构
[1] South Asian Univ, New Delhi 110021, India
[2] Natl Agri Food Biotechnol Inst NABI, Agri Biotechnol Div, Sas Nagar Mohali, Punjab, India
[3] Punjab Agr Univ, Ludhiana 141004, Punjab, India
[4] PU, UIET, Chandigarh 160014, India
基金
英国生物技术与生命科学研究理事会;
关键词
EXPRESSION; ARABIDOPSIS; TOLERANCE; GENES; TOOL; DNA;
D O I
10.1038/s41598-020-64746-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Heat shock proteins (HSPs) have a significant role in protein folding and are considered as prominent candidates for development of heat-tolerant crops. Understanding of wheat HSPs has great importance since wheat is severely affected by heat stress, particularly during the grain filling stage. In the present study, efforts were made to identify HSPs in wheat and to understand their role during plant development and under different stress conditions. HSPs in wheat genome were first identified by using Position-Specific Scoring Matrix (PSSMs) of known HSP domains and then also confirmed by sequence homology with already known HSPs. Collectively, 753 TaHSPs including 169 TaSHSP, 273 TaHSP40, 95 TaHSP60, 114 TaHSP70, 18 TaHSP90 and 84 TaHSP100 were identified in the wheat genome. Compared with other grass species, number of HSPs in wheat was relatively high probably due to the higher ploidy level. Large number of tandem duplication was identified in TaHSPs, especially TaSHSPs. The TaHSP genes showed random distribution on chromosomes, however, there were more TaHSPs in B and D sub-genomes as compared to the A sub-genome. Extensive computational analysis was performed using the available genomic resources to understand gene structure, gene expression and phylogentic relationship of TaHSPs. Interestingly, apart from high expression under heat stress, high expression of TaSHSP was also observed during seed development. The study provided a list of candidate HSP genes for improving thermo tolerance during developmental stages and also for understanding the seed development process in bread wheat.
引用
收藏
页数:12
相关论文
共 45 条
[1]   Cyanobacterial ClpC/HSP100 protein displays intrinsic chaperone activity [J].
Andersson, FI ;
Blakytny, R ;
Kirstein, J ;
Turgay, K ;
Bukau, B ;
Mogk, A ;
Clarke, AK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (09) :5468-5475
[2]   Shifting the limits in wheat research and breeding using a fully annotated reference genome [J].
Appels, Rudi ;
Eversole, Kellye ;
Feuillet, Catherine ;
Keller, Beat ;
Rogers, Jane ;
Stein, Nils ;
Pozniak, Curtis J. ;
Choulet, Frederic ;
Distelfeld, Assaf ;
Poland, Jesse ;
Ronen, Gil ;
Sharpe, Andrew G. ;
Pozniak, Curtis ;
Barad, Omer ;
Baruch, Kobi ;
Keeble-Gagnere, Gabriel ;
Mascher, Martin ;
Ben-Zvi, Gil ;
Josselin, Ambre-Aurore ;
Himmelbach, Axel ;
Balfourier, Francois ;
Gutierrez-Gonzalez, Juan ;
Hayden, Matthew ;
Koh, ChuShin ;
Muehlbauer, Gary ;
Pasam, Raj K. ;
Paux, Etienne ;
Rigault, Philippe ;
Tibbits, Josquin ;
Tiwari, Vijay ;
Spannagl, Manuel ;
Lang, Daniel ;
Gundlach, Heidrun ;
Haberer, Georg ;
Mayer, Klaus F. X. ;
Ormanbekova, Danara ;
Prade, Verena ;
Simkova, Hana ;
Wicker, Thomas ;
Swarbreck, David ;
Rimbert, Helene ;
Felder, Marius ;
Guilhot, Nicolas ;
Kaithakottil, Gemy ;
Keilwagen, Jens ;
Leroy, Philippe ;
Lux, Thomas ;
Twardziok, Sven ;
Venturini, Luca ;
Juhasz, Angela .
SCIENCE, 2018, 361 (6403) :661-+
[3]   MEME: discovering and analyzing DNA and protein sequence motifs [J].
Bailey, Timothy L. ;
Williams, Nadya ;
Misleh, Chris ;
Li, Wilfred W. .
NUCLEIC ACIDS RESEARCH, 2006, 34 :W369-W373
[4]   Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry [J].
Baranwal, Vinay Kumar ;
Negi, Nisha ;
Khurana, Paramjit .
SCIENTIFIC REPORTS, 2016, 6
[5]   The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana [J].
Cannon S.B. ;
Mitra A. ;
Baumgarten A. ;
Young N.D. ;
May G. .
BMC Plant Biology, 4 (1)
[6]   The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress [J].
Chauhan, Harsh ;
Khurana, Neetika ;
Nijhavan, Aashima ;
Khurana, Jitendra P. ;
Khurana, Paramjit .
PLANT CELL AND ENVIRONMENT, 2012, 35 (11) :1912-1931
[7]  
Conesa Ana, 2008, Int J Plant Genomics, V2008, P619832, DOI 10.1155/2008/619832
[8]   DEVELOPMENTAL CONTROL OF SMALL HEAT-SHOCK PROTEIN EXPRESSION DURING PEA SEED MATURATION [J].
DEROCHER, AE ;
VIERLING, E .
PLANT JOURNAL, 1994, 5 (01) :93-102
[9]   Intron gain, a dominant evolutionary process supporting high levels of gene expression in rice [J].
Deshmukh, Rupesh K. ;
Sonah, Humira ;
Singh, Nagendra K. .
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2016, 25 (02) :142-146
[10]   Protein folding in vivo:: the importance of molecular chaperones [J].
Feldman, DE ;
Frydman, J .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2000, 10 (01) :26-33