Polysaccharide-Protein Complex from Lycium barbarum L. Is a Novel Stimulus of Dendritic Cell Immunogenicity

被引：78

作者：

Chen, Zhisong ^{[2
,3
]}

Lu, Jinhua ^{[2
]}

Srinivasan, Nalini ^{[2
]}

Tan, Benny Kwong Huat ^{[3
]}

Chan, Soh Ha ^{[1
,2
]}

机构：

[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol, WHO,Collaborating Ctr Res & Training Immunol, Singapore 117597, Singapore

[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol & Immunol Programme, Singapore 117597, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Pharmacol, Singapore 117597, Singapore

来源：

JOURNAL OF IMMUNOLOGY | 2009年 / 182卷 / 06期

关键词：

TUMOR-NECROSIS-FACTOR; COLONY-STIMULATING FACTOR; BLOOD MONONUCLEAR-CELLS; MOUSE BONE-MARROW; ANTIGEN PRESENTATION; OXIDATIVE STRESS; FACTOR-ALPHA; FRUIT; MICE; PROLIFERATION;

D O I：

10.4049/jimmunol.0802567

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Dendritic cell (DC) immunogenicity correlates with its maturation, which can be induced by toxic microbial products such as LPS. In this study, we report that a nontoxic polysaccharide-protein complex isolated from a Chinese medicinal herb, Lycium barbarum (LBP), induces phenotypic and functional maturation of DCs with strong immunogenicity. LBP up-regulated DC expression of CD40, CD80, CD86, and MHC class H molecules; down-regulated DC uptake of Ag; enhanced DC allostimulatory activity; and induced IL-12p40 and p70 production. All of its five fractions were active. LBP developed enhanced Th1 response, and LBP-treated DCs enhanced Th1 and Th2 responses in vitro and in vivo. Our study provides evidence and rationale on using LBP in various clinical conditions to enhance host immunity and suggests LBP as a potent adjuvant for the design of DC-based vaccines. The Journal of Immunology, 2009, 182: 3503-3509.

引用

页码：3503 / 3509

页数：7

共 35 条

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