Validation of a fast real-time PCR method to detect fraud and mislabeling in milk and dairy products

被引：71

作者：

Di Domenico, M. ^{[1
]}

Di Giuseppe, M. ^{[1
]}

Rodriguez, J. D. Wicochea ^{[1
]}

Camma, C. ^{[1
]}

机构：

[1] Ist Zooprofilat Sperimentale Abruzzo & Molise G C, Ric & Sviluppo Biotecnol, Via Campo Boario, I-64100 Teramo, Italy

来源：

JOURNAL OF DAIRY SCIENCE | 2017年 / 100卷 / 01期

关键词：

species identification; dairy products; isoelectric focusing; real-time PCR; POLYMERASE-CHAIN-REACTION; WATER-BUFFALO MOZZARELLA; BOVINE-MILK; QUANTITATIVE DETECTION; GOATS MILK; SPECIES IDENTIFICATION; COWS MILK; SHEEPS MILK; CHEESE; QUANTIFICATION;

D O I：

10.3168/jds.2016-11695

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Fast real-time PCR TaqMan assays were developed and validated for species identification in dairy products. Based on the amplification of 12S rRNA and cytB partial genes of mitochondrial DNA, the methods were demonstrated to be sensitive, fast, and species-specific for Bos taurus, Ovis aries, Bubalus bubalis, and Capra hircus. The limit of detection calculated was lower than 1%, and the efficiency was reported to be higher than 96% in every assay. An internal amplification control was used to detect possible false negatives. The method was validated by means of laboratory-prepared samples mixing different species. Moreover, 18 commercial dairy samples were analyzed by both real-time PCR and isoelectric focusing, the official European Union reference method. The 4 TaqMan assays were confirmed to be a useful tool for milk and dairy product authentication.

引用

页码：106 / 112

页数：7

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