Dysregulation of Amyloid-β Protein Precursor, β-Secretase, Presenilin 1 and 2 Genes in the Rat Selectively Vulnerable CA1 Subfield of Hippocampus Following Transient Global Brain Ischemia

被引：58

作者：

Kocki, Janusz ^{[1
]}

Ulamek-Koziol, Marzena ^{[2
]}

Bogucka-Kocka, Anna ^{[3
]}

Januszewski, Slawomir ^{[2
]}

Jablonski, Miroslaw ^{[4
]}

Gil-Kulik, Paulina ^{[1
]}

Brzozowska, Judyta ^{[5
]}

Petniak, Alicja ^{[1
]}

Furmaga-Jablonska, Wanda ^{[6
]}

Bogucki, Jacek ^{[7
]}

Czuczwar, Stanislaw J. ^{[8
,9
]}

Pluta, Ryszard ^{[2
]}

机构：

[1] Med Univ Lublin, Dept Clin Genet, Lublin, Poland

[2] Polish Acad Sci, Mossakowski Med Res Ctr, Lab Ischem & Neurodegenerat Brain Res, Pawinskiego 5 Str, PL-02106 Warsaw, Poland

[3] Med Univ Lublin, Dept Pharmaceut Bot, Lublin, Poland

[4] Med Univ Lublin, Dept Rehabil & Orthopaed, Lublin, Poland

[5] Med Univ Lublin, Dept Clin Psychol, Lublin, Poland

[6] Med Univ Lublin, Dept Neonate & Infant Pathol, Lublin, Poland

[7] Warsaw Higher Humanist Sch, Warsaw, Poland

[8] Med Univ Lublin, Dept Pathophysiol, Lublin, Poland

[9] Inst Rural Med, Dept Physiopathol, Lublin, Poland

来源：

JOURNAL OF ALZHEIMERS DISEASE | 2015年 / 47卷 / 04期

关键词：

Alzheimer's disease; amyloid-beta protein precursor; beta-secretase; brain ischemia; CA1; area; dementia; gamma-secretase; genes; hippocampus; presenilin; 1; and; 2; DELAYED NEURONAL DEATH; CEREBRAL-ISCHEMIA; ALZHEIMERS-DISEASE; A-BETA; COGNITIVE IMPAIRMENT; REPERFUSION INJURY; APOLIPOPROTEIN-E; BARRIER; DAMAGE; NECROSIS;

D O I：

10.3233/JAD-150299

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The interaction between brain ischemia and Alzheimer's disease (AD) has been intensively investigated recently. Nevertheless, we have not yet understood the nature and mechanisms of the ischemic episodes triggering the onset of AD and how they influence its slow progression. The assumed connection between brain ischemia and the accumulation of amyloid-beta (A beta) peptide awaits to be clearly explained. In our research, we employed a rat cardiac arrest model to study the changes in gene expression of amyloid-beta protein precursor (A beta PP) and its cleaving enzymes, beta- and gamma-secretases (including presenilins) in hippocampal CA1 sector, following transient 10-min global brain ischemia. The quantitative reverse-transcriptase PCR assay demonstrated that the expression of all above genes that contribute to A beta peptide generation was dysregulated during 30 days in postischemic hippocampal CA1 area. It suggests that studied A beta peptide generation-related genes can be involved in A beta PP metabolism, following global brain ischemia and will be useful to identify the molecular mechanisms underpinning

引用

页码：1047 / 1056

页数：10