A diagnostic tool for efficient analysis of the population structure, hybridization and conservation status of European whitefish (Coregonus lavaretus (L.)) and vendace (C-albula (L.))

被引：14

作者：

Praebel, Kim ^{[1
]}

Westgaard, Jon-Ivar ^{[2
]}

Amundsen, Per-Arne ^{[1
]}

Siwertsson, Anna ^{[1
]}

Knudsen, Rune ^{[1
]}

Kahilainen, Kimmo K. ^{[3
,4
]}

Fevolden, Svein-Erik ^{[1
]}

机构：

[1] Univ Tromso, Dept Arctic & Marine Biol, N-9037 Tromso, Norway

[2] Inst Marine Res, Tromso Dept, N-9294 Tromso, Norway

[3] Univ Helsinki, Dept Environm Sci, FI-00014 Helsinki, Finland

[4] Univ Helsinki, Kilpisjarvi Biol Stn, FI-99490 Kilpisjarvi, Finland

来源：

BIOLOGY AND MANAGEMENT OF COREGONID FISHES - 2011 | 2013年 / 64卷

关键词：

conservation; Coregonus lavaretus; Coregonus albula; cross-species amplification; Microsatellite multiplex PCR; MICROSATELLITE LOCI; GENOTYPING ERRORS; DNA MARKERS; DIVERGENCE; INTROGRESSION; FISHERIES; ECOLOGY; NUMBERS;

D O I：

10.1127/1612-166X/2013/0064-0026

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Coregonids constitute one of the most diverse fish families in the northern freshwater systems and several species are highly endangered mainly due to anthropogenic pressure. Cost effective and powerful polymerase chain reaction (PCR) rnicrosatellite multiplex assays were established for genetic studies of the population structure, hybridization and conservation status of European whitefish (Coregonus lavaretus (L.)) and vendace (C. albula (L.)). An assay containing four PCR multiplexes for co-amplification of 9, 5, 3, and 4 loci was developed for C. lavaretus. Cross-species amplification and rearrangement of the same loci resulted in an assay containing three multiplex reactions of 6, 3, and 4 loci for C. albula. Highly significant pair-wise F-ST - estimates were obtained between C. albula from L. Vaggatem (Norway) and L. Palojarvi (Finland) (F-ST = 0.301, p < 0.001), between C. lavaretus from L. Skrukkebukta (Norway) and L. Stuorajavri (Norway) (F-ST = 0.161, p < 0.001), and between morphpairs occurring in the two latter lakes (F-ST = 0.0135-0.043, p < 0.001). The multiplex assays provided a 100% correct assignment success for discriminating C. lavaretus and C. albula and hence provide a powerful diagnostic tool for the future management of these species.

引用

页码：247 / 255

页数：9

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