Subunit a of cytochrome o oxidase requires both YidC and SecYEG for membrane insertion

被引：70

作者：

du Plessis, DJF ^{[1
]}

Nouwen, N ^{[1
]}

Driessen, AJM ^{[1
]}

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol, Dept Mol Microbiol, Inst Mat Sci Ctr Plus, NL-9751 NN Haren, Netherlands

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2006年 / 281卷 / 18期

关键词：

D O I：

10.1074/jbc.M600048200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that facilitate the insertion of membrane proteins. Depletion of YidC in E. coli leads to a specific defect in the functional assembly of major energy transducing complexes such as the F1F0 ATPase and cytochrome bo(3) oxidase. Here we report on the in vitro reconstitution of the membrane insertion of the CyoA subunit of cytochrome bo(3) oxidase. Efficient insertion of in vitro synthesized pre-CyoA into proteoliposomes requires YidC, SecYEG, and SecA and occurs independently of the proton motive force. These data demonstrate that pre-CyoA is a substrate of a novel pathway that involves both SecYEG and YidC.

引用

收藏

页码：12248 / 12252

页数：5

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