A Leaf Curl Disease in Germplasm of Rapeseed-Mustard in India: Molecular Evidence of a Weed-Infecting Begomovirus-Betasatellite Complex Emerging in a New Crop

被引：8

作者：

Roy, Anirban ^{[1
]}

Spoorthi, Poreddy ^{[1
]}

Bag, Manas Kumar ^{[1
]}

Prasad, Telaprolu Venkata ^{[1
]}

Singh, Ranbir ^{[1
]}

Dutta, Manoranjan ^{[1
]}

Mandal, Bikash ^{[2
]}

机构：

[1] Natl Bur Plant Genet Resources, Germplasm Evaluat Div, New Delhi 110012, India

[2] Indian Agr Res Inst, Div Plant Pathol, Adv Ctr Plant Virol, New Delhi 110012, India

来源：

JOURNAL OF PHYTOPATHOLOGY | 2013年 / 161卷 / 7-8期

关键词：

Betasatellite; Brassica spp; Croton yellow vein mosaic virus; India; Phylogenetic analysis; Rolling circle amplification; COMPLETE NUCLEOTIDE-SEQUENCE; VEIN MOSAIC DISEASE; DNA-BETA; DIVERSITY; VIRUS; GEMINIVIRUSES; RECOMBINATION; SATELLITES; TOMATO; HOST;

D O I：

10.1111/jph.12102

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Evaluation of 130 accessions of rapeseed-mustard germplasm grown at the National Bureau of Plant Genetic Resources, New Delhi, India during the winter season (2011-2012) revealed the occurrence of a leaf curl disease in seven accessions. The occurrence of the disease was observed in another 62 of 525 accessions evaluated during 2012-2013. The association of a monopartite begomovirus and betasatellite was established with the symptomatic plants by whitefly transmission and PCR amplification. The complete nucleotide sequences of the begomovirus (JX270684, 2745 nucleotides), obtained by rolling circle amplification, showed the highest sequence identity (98.1%) with the weed-infecting begomovirus, Croton yellow vein mosaic virus. Analysis of recombination indicated the probable occurrence of many overlapping inter- and intraspecific recombination events. The sequence of betasatellite (JX270685, 1355 nucleotides) showed the highest sequence identity (95.7%) with Croton yellow vein mosaic betasatellite. Begomoviruses were not previously known to naturally infect rapeseed-mustard. This is the first report of the emergence of a weed-infecting begomovirus-betasatellite complex in rapeseed-mustard germplasm in India and raises the concern on utilization of such susceptible germplasm in crop improvement programmes.

引用

页码：522 / 535

页数：14

共 38 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2]

Amin I., 2002, Plant Disease, V86, P444, DOI 10.1094/PDIS.2002.86.4.444B

[3] Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses [J].

Briddon, R. W. ;

Brown, J. K. ;

Moriones, E. ;

Stanley, J. ;

Zerbini, M. ;

Zhou, X. ;

Fauquet, C. M. .

ARCHIVES OF VIROLOGY, 2008, 153 (04) :763-781

[4] Universal primers for the PCR-mediated amplification of DNA β -: A molecule associated with some monopartite begomoviruses [J].

Briddon, RW ;

Bull, SE ;

Mansoor, S ;

Amin, I ;

Markham, PG .

MOLECULAR BIOTECHNOLOGY, 2002, 20 (03) :315-318

[5] A simple and robust statistical test for detecting the presence of recombination [J].

Bruen, TC ;

Philippe, H ;

Bryant, D .

GENETICS, 2006, 172 (04) :2665-2681

[6] Neighbor-Net: An agglomerative method for the construction of phylogenetic networks [J].

Bryant, D ;

Moulton, V .

MOLECULAR BIOLOGY AND EVOLUTION, 2004, 21 (02) :255-265

[7]

Doyle JJ., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362

[8]

Edwardson J.R., 1991, FLORIDA AGR EXPT STA, V16, P1244

[9] Geminivirus strain demarcation and nomenclature [J].

Fauquet, C. M. ;

Briddon, R. W. ;

Brown, J. K. ;

Moriones, E. ;

Stanley, J. ;

Zerbini, M. ;

Zhou, X. .

ARCHIVES OF VIROLOGY, 2008, 153 (04) :783-821

[10] Founder effect, plant host, and recombination shape the emergent population of begomoviruses that cause the tomato yellow leaf curl disease in the Mediterranean basin [J].

Garcia-Andres, Susana ;

Accotto, Gian Paolo ;

Navas-Castillo, Jesus ;

Moriones, Enrique .

VIROLOGY, 2007, 359 (02) :302-312

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