Organotypic Culture of Full-thickness Adult Porcine Retina

被引：14

作者：

Wang, Jianfeng ^{[1
]}

Kolomeyer, Anton M. ^{[2
]}

Zarbin, Marco A. ^{[2
]}

Townes-Anderson, Ellen ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Newark, NJ 07103 USA

[2] Univ Med & Dent New Jersey, Inst Ophthalmol & Visual Sci, Newark, NJ 07103 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2011年 / 49期

关键词：

In vitro; Issue; 49; Neuroscience; Photoreceptor; Porcine; Retina;

D O I：

10.3791/2655

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

There is a recognized demand for in vitro models that can replace or reduce animal experiments. Porcine retina has a similar neuronal structure to human retina and is therefore a valuable species for studying mechanisms of human retinal injury and degenerative disease. Here we describe a cost-effective technique for organotypic culture of adult porcine retina isolated from eyes obtained from an abattoir. After removing the anterior segment, a trephine blade was used to create multiple neural retina-Bruch's membrane-RPE-choroid-sclera explants from the posterior segment of adult porcine eyes. A piece of sterile filter paper was used to lift the neural retina off from each explant. The filter paper-retina complex was cultured (photoreceptor side up) atop an insert, which was held away from the bottom of the culture dish by a custom-made stand. The stand allows for good circulation of the culture medium to both sides of the retina. Overall, this procedure is simple, reproducible, and permits preservation of native retinal structure for at least seven days, making it a useful model for a variety of morphological, pharmacological, and biochemical studies on mammalian retina. © JoVE 2006-2011 All Rights Reserved.

引用

页数：1

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