Characterization of surface-confined α-synuclein by surface plasmon resonance measurements

被引：15
|
作者：
Kang, T [1 ]
Hong, S [1 ]
Kim, HJ [1 ]
Moon, J [1 ]
Oh, S [1 ]
Paik, SR [1 ]
Yi, J [1 ]
机构：
[1] Seoul Natl Univ, Sch Chem & Biol Engn, Inst Chem Proc, Seoul 151742, South Korea
来源：
LANGMUIR | 2006年 / 22卷 / 01期
关键词：
D O I：
10.1021/la052276w
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Urea-driven denaturation and renaturation of surface-bound alpha-synuclein are monitored by surface plasmon resonance (SPR) spectroscopy. The differential SPR angle shift (Delta Theta(SPR))(Net) enables us to estimate the Gibbs free energy change (Delta G degrees) for the denaturation of the supported alpha-synuclein. Delta G degrees for the denaturation of the supported alpha-synuclein, which is indirectly related to its biological activity can be increased significantly by the mixed self-assembled monolayers of 11-mercaptoundecanoic acid and 1,6-hexanedithiol. These SPR measurements of surface-bound biomolecules suggested herein can be further utilized to design effective biological scaffold for biosensor, biocatalyst, and possible diagnosis.
引用
收藏
页码：13 / 17
页数：5
相关论文
共 50 条
  • [21] Surface Plasmon Resonance for Crude Oil Characterization
    Ooms, Matthew D.
    Fadaei, Hossein
    Sinton, David
    ENERGY & FUELS, 2015, 29 (05) : 3019 - 3023
  • [22] On the stability of surface-confined nanoporous molecular networks
    Ghijsens, Elke
    Adisoejoso, Jinne
    Van Gorp, Hans
    Destoop, Iris
    Noguchi, Aya
    Ivasenko, Oleksandr
    Tahara, Kazukuni
    Van der Auweraer, Mark
    Tobe, Yoshito
    De Feyter, Steven
    JOURNAL OF CHEMICAL PHYSICS, 2015, 142 (10):
  • [23] Materials engineering for surface-confined flame retardancy
    Alongi, Jenny
    Carosio, Federico
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 252
  • [24] Surface-Confined Electrochemiluminescence Microscopy of Cell Membranes
    Sojic, Neso (neso.sojic@enscbp.fr), 1600, American Chemical Society (140):
  • [25] Amplification of chirality in surface-confined supramolecular bilayers
    Hai Cao
    Steven De Feyter
    Nature Communications, 9
  • [26] Materials engineering for surface-confined flame retardancy
    Malucelli, Giulio
    Carosio, Federico
    Alongi, Jenny
    Fina, Alberto
    Frache, Alberto
    Camino, Giovanni
    MATERIALS SCIENCE & ENGINEERING R-REPORTS, 2014, 84 : 1 - 20
  • [27] Phases of surface-confined trivalent colloidal particles
    Swinkels, Piet J. M.
    Gong, Zhe
    Sacanna, Stefano
    Noya, Eva G.
    Schall, Peter
    SOFT MATTER, 2023, 19 (19) : 3414 - 3422
  • [28] Tailoring Surface-Confined Nanopores with Photoresponsive Groups
    Tahara, Kazukuni
    Inukai, Koji
    Adisoejoso, Jinne
    Yamaga, Hiroyuki
    Balandina, Tatyana
    Blunt, Matthew O.
    De Feyter, Steven
    Tobe, Yoshito
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (32) : 8373 - 8376
  • [29] Polyelectrolyte adsorption onto a surface-confined surfactant
    Artyukhin, AB
    Burnham, KJ
    Levchenko, AA
    Talroze, RV
    Stroeve, P
    LANGMUIR, 2003, 19 (06) : 2243 - 2248
  • [30] Copper deposition in the presence of surface-confined additives
    Univ of Illinois at Urbana-Champaign, Urbana, United States
    J Electrochem Soc, 1 (96-105):
12345