Urocortins and CRF type 2 receptor isoforms expression in the rat stomach are regulated by endotoxin: role in the modulation of delayed gastric emptying

被引:20
作者
Yuan, Pu-Qing [1 ]
Wu, S. Vincent
Tache, Yvette
机构
[1] Univ Calif Los Angeles, Ctr Neurobiol Stress, Div Digest Dis, CURE DDRC,VA GLAHS,Dept Med, Los Angeles, CA 90073 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2012年 / 303卷 / 01期
关键词
Astressin(2)-B; laser capture microdissection; lipopolysaccharide; myenteric neurons; upper gut; CORTICOTROPIN-RELEASING-FACTOR; MESSENGER-RIBONUCLEIC-ACID; ENTERIC NERVOUS-SYSTEM; FASTED PLASMA GHRELIN; TNF-ALPHA RELEASE; GASTROINTESTINAL-TRACT; PERIPHERAL UROCORTIN; SPLICE VARIANT; NITRIC-OXIDE; II GENE;
D O I
10.1152/ajpgi.00547.2011
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Yuan PQ, Wu SV, Tache Y. Urocortins and CRF type 2 receptor isoforms expression in the rat stomach are regulated by endotoxin: role in the modulation of delayed gastric emptying. Am J Physiol Gastrointest Liver Physiol 303: G20-G31, 2012. First published April 19, 2012; doi:10.1152/ajpgi.00547.2011.-Peripheral activation of corticotropin-releasing factor receptor type 2 (CRF2) by urocortin 1, 2, or 3 (Ucns) exerts powerful effects on gastric function; however, little is known about their expression and regulation in the stomach. We investigated the expression of Ucns and CRF2 isoforms by RT-PCR in the gastric corpus (GC) mucosa and submucosa plus muscle (S + M) or laser captured layers in naive rats, their regulations by lipopolysaccharide (LPS, 100 mu g/kg ip) over 24 h, and the effect of the CRF2 antagonist astresssin(2)-B (100 mu g/kg sc) on LPS-induced delayed gastric emptying (GE) 2-h postinjection. Transcripts of Ucns and CRF2b, the most common wild-type CRF2 isoform in the periphery, were expressed in all layers, including myenteric neurons. LPS increased Ucn mRNA levels significantly in both mucosa and S + M, reaching a maximal response at 6 h postinjection and returning to basal levels at 24 h except for Ucn 1 in S + M. By contrast, CRF2b mRNA level was significantly decreased in the mucosa and M + S with a nadir at 6 h. In addition, CRF2a, reportedly only found in the brain, and the novel splice variant CRF2a-3 were also detected in the GC, antrum, and pylorus. LPS reciprocally regulated these variants with a decrease of CRF2a and an increase of CRF2a-3 in the GC 6 h postinjection. Astressin(2)-B exacerbated LPS-delayed GE (42-73%, P < 0.001). These data indicate that Ucn and CRF2 isoforms are widely distributed throughout the rat stomach and inversely regulated by immune stress. The CRF2 signaling system may act to counteract the early gastric motor alterations to endotoxemia.
引用
收藏
页码:G20 / G31
页数:12
相关论文
共 71 条
  • [1] Corticosteroid-independent inhibition of tumor necrosis factor production by the neuropeptide urocortin
    Agnello, D
    Bertini, R
    Sacco, S
    Meazza, C
    Villa, P
    Ghezzi, P
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1998, 275 (05): : E757 - E762
  • [2] Regulation of type-2 corticotropin-releasing hormone receptor mRNA in rat heart by glucocorticoids and urocortin
    Asaba, K
    Makino, S
    Nishiyama, M
    Hashimoto, K
    [J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2000, 36 (04) : 493 - 497
  • [3] Urocrotin reduces food intake and gastric emptying in lean and ob/ob obese mice
    Asakawa, A
    Inui, A
    Ueno, N
    Makino, S
    Fujino, MA
    Kasuga, W
    [J]. GASTROENTEROLOGY, 1999, 116 (06) : 1287 - 1292
  • [4] Bacterial lipopolysaccharide shifts fasted plasma ghrelin to postprandial levels in rats
    Basa, NR
    Wang, LX
    Arteaga, JR
    Heber, D
    Livingston, EH
    Taché, Y
    [J]. NEUROSCIENCE LETTERS, 2003, 343 (01) : 25 - 28
  • [5] Bittencourt JC, 1999, J COMP NEUROL, V415, P285
  • [6] Endotoxin inhibits gastric emptying in rats via a capsaicin-sensitive afferent pathway
    Calatayud, S
    Barrachina, MD
    García-Zaragozá, E
    Quintana, E
    Esplugues, JV
    [J]. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2001, 363 (03) : 276 - 280
  • [7] Urocortin 2 expression in the rat gastrointestinal tract under basal conditions and in chemical colitis
    Chang, Jen
    Hoy, Julia J.
    Idumalla, Prema S.
    Clifton, Matthew S.
    Pecoraro, Norman C.
    Bhargava, Aditi
    [J]. PEPTIDES, 2007, 28 (07) : 1453 - 1460
  • [8] Urocortin 1 modulates immunosignaling in a rat model of colitis via corticotropin-releasing factor receptor 2
    Chang, Jen
    Adams, Melanie R.
    Clifton, Matthew S.
    Liao, Min
    Brooks, Julia H.
    Hasdemir, Burcu
    Bhargava, Aditi
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2011, 300 (05): : G884 - G894
  • [9] Differential profile of CRF receptor distribution in the rat stomach and duodenum assessed by newly developed CRF receptor antibodies
    Chatzaki, E
    Murphy, BJ
    Wang, LX
    Million, M
    Ohning, GV
    Crowe, PD
    Petroski, R
    Taché, Y
    Grigoriadis, DE
    [J]. JOURNAL OF NEUROCHEMISTRY, 2004, 88 (01) : 1 - 11
  • [10] Urocortin in human gastric mucosa: Relationship to inflammatory activity
    Chatzaki, E
    Charalampopoulos, I
    Leontidis, C
    Mouzas, IA
    Tzardi, M
    Tsatsanis, C
    Margioris, AN
    Gravanis, A
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2003, 88 (01) : 478 - 483