Molecular Characteristics of Extended-Spectrum Beta-Lactamases and qnr Determinants in Enterobacter Species from Japan

被引：25

作者：

Kanamori, Hajime ^{[1
]}

Yano, Hisakazu ^{[2
]}

Hirakata, Yoichi ^{[2
]}

Hirotani, Ayako ^{[1
]}

Arai, Kazuaki ^{[2
]}

Endo, Shiro ^{[1
]}

Ichimura, Sadahiro ^{[3
]}

Ogawa, Miho ^{[3
]}

Shimojima, Masahiro ^{[3
]}

Aoyagi, Tetsuji ^{[1
]}

Hatta, Masumitsu ^{[1
]}

Yamada, Mitsuhiro ^{[4
]}

Gu, Yoshiaki ^{[4
]}

Tokuda, Koichi ^{[1
]}

Kunishima, Hiroyuki ^{[4
]}

Kitagawa, Miho ^{[1
]}

Kaku, Mitsuo ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Dept Infect Control & Lab Diagnost, Sendai, Miyagi 980, Japan

[2] Tohoku Univ, Grad Sch Med, Dept Clin Microbiol Epidemiol Res & Management &, Sendai, Miyagi 980, Japan

[3] BML Inc, Dept Bacteriol, Kawagoe, Saitama, Japan

[4] Tohoku Univ, Grad Sch Med, Dept Reg Cooperat Infect Dis, Sendai, Miyagi 980, Japan

来源：

PLOS ONE | 2012年 / 7卷 / 06期

基金：

日本学术振兴会;

关键词：

MEDIATED QUINOLONE RESISTANCE; KLEBSIELLA-PNEUMONIAE; CITROBACTER-FREUNDII; SERRATIA-MARCESCENS; ESCHERICHIA-COLI; CLOACAE; PLASMID; PREVALENCE; DISSEMINATION; HOSPITALS;

D O I：

10.1371/journal.pone.0037967

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The incidence of extended-spectrum beta-lactamases (ESBLs) has been increasing worldwide, but screening criteria for detection of ESBLs are not standardized for AmpC-producing Enterobacteriaceae such as Enterobacter species. In this study, we investigated the prevalence of ESBLs and/or AmpC beta-lactamases in Japanese clinical isolates of Enterobacter spp. and the association of plasmid-mediated quinolone resistance (PMQR) determinants with ESBL producers. A total of 364 clinical isolates of Enterobacter spp. collected throughout Japan between November 2009 and January 2010 were studied. ESBL-producing strains were assessed by the CLSI confirmatory test and the boronic acid disk test. PCR and sequencing were performed to detect CTX-M, TEM, and SHV type ESBLs and PMQR determinants. For ESBL-producing Enterobacter spp., pulsed-field gel electrophoresis (PFGE) was performed using XbaI restriction enzyme. Of the 364 isolates, 22 (6.0%) were ESBL producers. Seven isolates of Enterobacter cloacae produced CTX-M-3, followed by two isolates producing SHV-12. Two isolates of Enterobacter aerogenes produced CTX-M-2. Of the 22 ESBL producers, 21 had the AmpC enzyme, and six met the criteria for ESBL production in the boronic acid test. We found a significant association of qnrS with CTX-M-3-producing E. cloacae. The 11 ESBL-producing Enterobacter spp. possessing bla(CTX-M), bla(SHV), or bla(TEM) were divided into six unique PFGE types. This is the first report about the prevalence of qnr determinants among ESBL-producing Enterobacter spp. from Japan. Our results suggest that ESBL-producing Enterobacter spp. with qnr determinants are spreading in Japan.

引用

页数：5

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