Mechanisms underlying cytotoxicity of anti-DNA antibodies

被引:5
作者
Suchkov, SV [1 ]
机构
[1] Moscow Reg Clin Inst, Clin Immunol Lab, Moscow, Russia
关键词
DNA abzymes; cytotoxicity; apoptosis; autoimmunity;
D O I
10.1023/A:1017936317842
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We studied mechanisms underlying cytotoxicity of catalytic anti-DNA autoantibodies or DNA abzymes isolated from the blood Of patients with systemic lupus erythematosus and autoimmune mice. Experiments were performed on L929, HL-60, Raji, and K562 cells. Treatment with DNA abzymes caused internucleosomal DNA fragmentation in target cells typical of apoptosis. Direct immunofluorescence assay demonstrated that DNA abzymes can penetrate into the nucleus of target cells. The dependence of antibody-mediated cytotoxicity on the duration of incubation indicated that cell death is realized via at least 2 mechanisms: penetration of DNA abzymes into the cell nucleus followed by degradation of nuclear DNA or induction of apoptosis.
引用
收藏
页码:475 / 478
页数:4
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