Profiling of metabolome and bacterial community dynamics in ensiled Medicago sativa inoculated without or with Lactobacillus plantarum or Lactobacillus buchneri

被引：145
作者：
Guo, X. S. [1 ,2 ]
Ke, W. C. [1 ,2 ]
Ding, W. R. [1 ,2 ]
Ding, L. M. [1 ,2 ]
Xu, D. M. [1 ,2 ]
Wang, W. W. [1 ]
Zhang, P. [1 ,2 ]
Yang, F. Y. [3 ]
机构：
[1] Lanzhou Univ, Sch Life Sci, State Key Lab Grassland & Agroecosyst, Lanzhou 730000, Gansu, Peoples R China
[2] Lanzhou Univ, Probiot & Biol Feed Res Ctr, Lanzhou 730000, Gansu, Peoples R China
[3] China Agr Univ, Coll Anim Sci & Technol, Inst Grassland Sci, Beijing 100193, Peoples R China
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
基金：
中国国家自然科学基金;
关键词：
LACTIC-ACID BACTERIA; AEROBIC STABILITY; ALFALFA SILAGE; FERMENTATION; GRASS; CORN;
D O I：
10.1038/s41598-017-18348-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Using gas chromatography mass spectrometry and the PacBio single molecule with real-time sequencing technology (SMRT), we analyzed the detailed metabolomic profiles and microbial community dynamics involved in ensiled Medicago sativa (alfalfa) inoculated without or with the homofermenter Lactobacillus plantarum or heterofermenter Lactobacillus buchneri. Our results revealed that 280 substances and 102 different metabolites were present in ensiled alfalfa. Inoculation of L. buchneri led to remarkable up-accumulation in concentrations of 4-aminobutyric acid, some free amino acids, and polyols in ensiled alfalfa, whereas considerable down-accumulation in cadaverine and succinic acid were observed in L. plantarum-inoculated silages. Completely different microbial flora and their successions during ensiling were observed in the control and two types of inoculant-treated silages. Inoculation of the L. plantarum or L. buchneri alters the microbial composition dynamics of the ensiled forage in very different manners. Our study demonstrates that metabolomic profiling analysis provides a deep insight in metabolites in silage. Moreover, the PacBio SMRT method revealed the microbial composition and its succession during the ensiling process at the species level. This provides information regarding the microbial processes underlying silage formation and may contribute to target-based regulation methods to achieve high-quality silage production.
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页数：10
相关论文
共 37 条
  • [21] Bacterial population dynamics during the ensiling of Medicago sativa (alfalfa) and subsequent exposure to air
    McGarvey, J. A.
    Franco, R. B.
    Palumbo, J. D.
    Hnasko, R.
    Stanker, L.
    Mitloehner, F. M.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2013, 114 (06) : 1661 - 1670
  • [22] Efficacy of a 3rd generation high-throughput sequencing platform for analyses of 16S rRNA genes from environmental samples
    Mosher, Jennifer J.
    Bernberg, Erin L.
    Shevchenko, Olga
    Kan, Jinjun
    Kaplan, Louis A.
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 95 (02) : 175 - 181
  • [23] Silage microbiology and its control through additives
    Muck, Richard E.
    [J]. REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, 2010, 39 : 183 - 191
  • [24] Biogenic amine production in grass, maize and total mixed ration silages inoculated with Lactobacillus casei or Lactobacillus buchneri
    Nishino, N.
    Hattori, H.
    Wada, H.
    Touno, E.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2007, 103 (02) : 325 - 332
  • [25] Interactive metagenomic visualization in a Web browser
    Ondov, Brian D.
    Bergman, Nicholas H.
    Phillippy, Adam M.
    [J]. BMC BIOINFORMATICS, 2011, 12
  • [26] Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass, whole crop maize, guinea grass and rhodes grass silages
    Parvin, S.
    Wang, C.
    Li, Y.
    Nishino, N.
    [J]. ANIMAL FEED SCIENCE AND TECHNOLOGY, 2010, 160 (3-4) : 160 - 166
  • [27] Bacterial community associated with ensilage process of wilted guinea grass
    Parvin, S.
    Nishino, N.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2009, 107 (06) : 2029 - 2036
  • [28] Pouliot-Mathieu Kat, 2013, PharmaNutrition, V1, P141, DOI 10.1016/j.phanu.2013.06.003
  • [29] Effects of combining Lactobacillus buchneri 40788 with various lactic acid bacteria on the fermentation and aerobic stability of corn silage
    Reich, Lindsey J.
    Kung, Limin, Jr.
    [J]. ANIMAL FEED SCIENCE AND TECHNOLOGY, 2010, 159 (3-4) : 105 - 109
  • [30] The development of lactic acid bacteria and Lactobacillus buchneri and their effects on the fermentation of alfalfa silage
    Schmidt, R. J.
    Hu, W.
    Mills, J. A.
    Kung, L., Jr.
    [J]. JOURNAL OF DAIRY SCIENCE, 2009, 92 (10) : 5005 - 5010
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