Molecular Detection of Rifabutin-Susceptible Mycobacterium tuberculosis

被引：17

作者：

Chen, Huang-Yao ^{[1
]}

Yu, Ming-Chih ^{[2
]}

Huang, Wei-Lun ^{[1
]}

Wu, Mei-Hua ^{[1
]}

Chang, Yung-Lin ^{[1
]}

Che, Chien-Rai ^{[1
]}

Jou, Ruwen ^{[1
,3
]}

机构：

[1] Ctr Dis Control, Reference Lab Mycobacteriol, Res & Diagnost Ctr, Taipei, Taiwan

[2] Taipei Med Univ, Wan Fang Hosp, Dept Internal Med, Div Pulm Med, Taipei, Taiwan

[3] Natl Yang Ming Univ, Inst Microbiol & Immunol, Taipei 112, Taiwan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 06期

关键词：

CROSS-RESISTANCE; RPOB MUTATIONS; STRAINS; RIFAMPICIN; GENE;

D O I：

10.1128/JCM.00652-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rapid assays are still needed to detect rifabutin (RFB) susceptibility for proper tuberculosis treatment. To assess the use of the GenoType MTBDRplus assay and subsequent rpoB gene sequencing on detection of RFB susceptibility, we analyzed 800 multi-drug-resistant Mycobacterium tuberculosis isolates, and 13% (104/800) were RFB susceptible. Of the 104 RFB-susceptible isolates, 71 (68.3%) isolates were rapidly identified using two molecular assays, while the remaining isolates could be determined using conventional drug-susceptibility testing according to the clinician's decision.

引用

页码：2085 / 2088

页数：4

共 13 条

[1]

Chikamatsu Kinuyo, 2009, Kekkaku, V84, P631

[2]

Hain Lifescience GmbH, 2009, GENOTYPE MTBDRPLUS I

[3] Comparison of two commercial assays for the characterization of rpoB mutations in Mycobacterium tuberculosis and description of new mutations conferring weak resistance to rifampicin [J].

Hauck, Yolande ;

Fabre, Michel ;

Vergnaud, Gilles ;

Soler, Charles ;

Pourcel, Christine .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 64 (02) :259-262

[4] Mutations in the beginning of the rpoB gene can induce resistance to rifamycins in both Helicobacter pylori and Mycobacterium tuberculosis [J].

Heep, M ;

Rieger, U ;

Beck, D ;

Lehn, N .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (04) :1075-1077

[5] Performance Assessment of the GenoType MTBDRplus Test and DNA Sequencing in Detection of Multidrug-Resistant Mycobacterium tuberculosis [J].

Huang, Wei-Lun ;

Chen, Huang-Yau ;

Kuo, Yuh-Min ;

Jou, Ruwen .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (08) :2520-2524

[6] Genetic diversity of multidrug-resistant Mycobacterium tuberculosis isolates and identification of 11 novel rpoB alleles in Taiwan [J].

Jou, R ;

Chen, HY ;

Chiang, CY ;

Yu, MC ;

Su, IJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (03) :1390-1394

[7]

Ling Daphne I, 2008, Expert Rev Respir Med, V2, P583, DOI 10.1586/17476348.2.5.583

[8] IN-VITRO AND INTRACELLULAR ACTIVITY OF RIFABUTIN ON DRUG-SUSCEPTIBLE AND MULTIPLE DRUG-RESISTANT (MDR) TUBERCLE-BACILLI [J].

LUNAHERRERA, J ;

REDDY, MV ;

GANGADHARAM, PRJ .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1995, 36 (02) :355-363

[9]

National Committee for Clinical Laboratory Standards, 2000, M24A NAT COMM CLIN L, V23

[10] Incidence of cross resistance between rifampicin and rifabutin in Mycobacterium tuberculosis strains in Izmir, Turkey [J].

Senol, G ;

Erbaycu, A ;

Özsöz, A .

JOURNAL OF CHEMOTHERAPY, 2005, 17 (04) :380-384

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