Blotting patterns of IgG anti-(U1)RNP antibodies in mixed connective tissue disease

被引:14
作者
Ghirardello, A
Doria, A
Vesco, P
Vaccaro, E
Bernardi, C
Catani, C
Fagiolo, U
Gambari, PF
机构
[1] UNIV PADUA POLYCLIN,IST MED INTERNA,CATTEDRA REUMATOL,I-35128 PADUA,ITALY
[2] UNIV PADUA,INST INTERNAL MED,DIV RHEUMATOL,PADUA,ITALY
[3] UNIV PADUA,DEPT BIOCHEM SCI,CNR,UNIT MUSCLE BIOL & PHYSIOPATHOL,PADUA,ITALY
关键词
anti-(U1)RNP antibody; mixed connective tissue disease; immunoblotting; small nuclear ribonucleoprotein; epitope specificity;
D O I
10.1007/BF01419727
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Serum reactivities towards individual U1 snRNP proteins were determined by immunoblotting in 32 patients with mixed connective tissue disease (MCTD). Time persistence of immunoblot profiles and clinical significance of anti-(U1)RNP antibody specificities were also investigated. IgG anti-(U1)RNP antibodies were found in the sera of 29 out of 32 patients (90.6%): 21 (65.6%) reacted with the 70-kD protein, 25 (78.1%) with A, 23 (71.9%) with C and 20 (62.5%) with B/B' proteins. None were reactive with the Sm-D peptide. Seventy kilodalton antibody specificity was strongly associated with a higher antinuclear antibody titre (>160) and slightly associated with disease activity; anti-B/B' specificity was associated with lymphadenopathy. Anti-A, -C and -B/B' antibodies were negatively associated with systemic lupus erythematosus (SLE) skin rashes. Two types of anti-(U1)RNP blotting patterns were selected: ''full spectrum'' (53.1% of cases) and a ''partially/no reactive'' one (46.9%). Such patterns were unchanged over time in 14 out of 16 cases prospectively examined(87.5%), while the pattern shifted from ''full spectrum'' to ''partially/no reactive'' in 2 cases (12.5%): in 1 after a prolonged clinical remission (24 years) and in the other following immunosuppressive therapy. The anti- (U1)RNP antibody immunoblot profile in MCTD patients consisted of various reactivities and remained unchanged over time in most cases. Antibody reactivity against the 70-kD protein represented the major U1 snRNP specificity. The various anti-(U1)RNP specific reactivities demonstrated poor clinical significance within MCTD. Thus, MCTD seems to be characterized by a longstanding serological heterogeneity whose reactivities do not apparently correspond to distinct features within the broad clinical spectrum of MCTD.
引用
收藏
页码:145 / 150
页数:6
相关论文
共 33 条
[1]  
Alarcon-Segovia D., 1987, Mixed Connective Tissue Disease and Anti-Nuclear Antibodies, P33
[2]  
ALARCONSEGOVIA D, 1989, J RHEUMATOL, V16, P328
[3]   POLYMYOSITIS AND DERMATOMYOSITIS .1. [J].
BOHAN, A ;
PETER, JB .
NEW ENGLAND JOURNAL OF MEDICINE, 1975, 292 (07) :344-347
[4]  
BUNN CC, 1982, J CLIN LAB IMMUNOL, V8, P13
[5]  
COMBE B, 1989, CLIN EXP IMMUNOL, V75, P18
[6]  
COTES JE, 1979, LUNG FUNCTION
[7]   USE OF RECOMBINANT RNP PEPTIDE-70K AND PEPTIDE-A IN AN ELISA FOR MEASUREMENT OF ANTIBODIES IN MIXED CONNECTIVE-TISSUE DISEASE - A LONGITUDINAL FOLLOW-UP OF 18 PATIENTS [J].
DEROOIJ, DJRAM ;
HABETS, WJ ;
VANDEPUTTE, LBA ;
HOET, MH ;
VERBEEK, AL ;
VANVENROOIJ, WJ .
ANNALS OF THE RHEUMATIC DISEASES, 1990, 49 (06) :391-395
[8]  
DORIA A, 1991, J RHEUMATOL, V18, P685
[9]  
DORIA A, 1991, J RHEUMATOL, V19, P259
[10]  
HABETS WJ, 1983, CLIN EXP IMMUNOL, V54, P265