Synthetic biology in mammalian cells: next generation research tools and therapeutics

被引：228

作者：

Lienert, Florian ^{[1
]}

Lohmueller, Jason J. ^{[1
]}

Garg, Abhishek ^{[1
]}

Silver, Pamela A. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2014年 / 15卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

PROTEIN-PROTEIN INTERACTIONS; CRE DRIVER LINES; GENE-EXPRESSION; IN-VITRO; INTRACELLULAR DELIVERY; SYSTEMATIC DISSECTION; MEDIATED DELIVERY; LOGIC COMPUTATION; T-CELLS; RNA;

D O I：

10.1038/nrm3738

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent progress in DNA manipulation and gene circuit engineering has greatly improved our ability to programme and probe mammalian cell behaviour. These advances have led to a new generation of synthetic biology research tools and potential therapeutic applications. Programmable DNA-binding domains and RNA regulators are leading to unprecedented control of gene expression and elucidation of gene function. Rebuilding complex biological circuits such as T cell receptor signalling in isolation from their natural context has deepened our understanding of network motifs and signalling pathways. Synthetic biology is also leading to innovative therapeutic interventions based on cell-based therapies, protein drugs, vaccines and gene therapies.

引用

页码：95 / 107

页数：13

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