Poly(ε-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells

被引：82

作者：

Crowder, Spencer W. ^{[1
,2
]}

Liang, Yi ^{[1
]}

Rath, Rutwik ^{[1
,2
]}

Park, Andrew M. ^{[3
]}

Maltais, Simon ^{[4
]}

Pintauro, Peter N. ^{[3
]}

Hofmeister, William ^{[5
]}

Lim, Chee C. ^{[6
]}

Wang, Xintong ^{[1
,2
]}

Sung, Hak-Joon ^{[1
,2
,6
]}

机构：

[1] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Med Ctr, Ctr Stem Cell Biol, Nashville, TN USA

[3] Vanderbilt Univ, Dept Chem & Biomol Engn, Nashville, TN 37235 USA

[4] Vanderbilt Univ, Med Ctr, Dept Cardiac Surg & Cardiol, Nashville, TN USA

[5] Univ Tennessee, Inst Space, Ctr Laser Applicat, Tullahoma, TN 37388 USA

[6] Vanderbilt Univ, Med Ctr, Dept Med, Nashville, TN USA

来源：

NANOMEDICINE | 2013年 / 8卷 / 11期

基金：

美国国家科学基金会;

关键词：

carbon nanotube; cardiac differentiation; electrical conductivity; mesenchymal stem cell; poly(epsilon-caprolactone); CARBON NANOTUBES; CARDIOMYOCYTE PHENOTYPE; GROWTH;

D O I：

10.2217/nnm.12.204

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs). Materials & methods: Electrospun scaffolds of poly(epsilon-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs. Results: Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(epsilon-caprolactone) scaffolds without carbon nanotube and azacytidine. Conclusion: This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium. Original submitted 23 July 2012; Revised submitted 31 October 2012; Published online 27 March 2013

引用

页码：1763 / 1776

页数：14

共 35 条

[1] Identification of cardiomyogenic lineage markers in untreated human bone marrow-derived mesenchymal stem cells [J].

Bayes-Genis, A ;

Roura, S ;

Soler-Botija, C ;

Farré, J ;

Hove-Madsen, L ;

Llach, A ;

Cinca, J .

TRANSPLANTATION PROCEEDINGS, 2005, 37 (09) :4077-4079

[2] Microstructure and cytocompatibility of electrospun nanocomposites based on poly(ε-caprolactone) and carbon nanostructures [J].

Bianco, Alessandra ;

Del Gaudio, Costantino ;

Baiguera, Silvia ;

Armentano, Ilaria ;

Bertarelli, Chiara ;

Dottori, Mariaserena ;

Bultrini, Giorgio ;

Lucotti, Andrea ;

Maria Kenny, Jose ;

Folin, Marcella .

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 2010, 33 (05) :271-282

[3] Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation [J].

Chen, Silvia S. ;

Fitzgerald, Wendy ;

Zimmerberg, Joshua ;

Kleinman, Hynda K. ;

Margolis, Leonid .

STEM CELLS, 2007, 25 (03) :553-561

[4] The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation [J].

Christopherson, Gregory T. ;

Song, Hongjun ;

Mao, Hai-Quan .

BIOMATERIALS, 2009, 30 (04) :556-564

[5]

Clark MD, 2009, J PHYS CHEM C, V113, P20861, DOI [10.1021/jp907221g, 10.1021/jp907221]

[6] Modular polymer design to regulate phenotype and oxidative response of human coronary artery cells for potential stent coating applications [J].

Crowder, Spencer W. ;

Gupta, Mukesh K. ;

Hofmeister, Lucas H. ;

Zachman, Angela L. ;

Sung, Hak-Joon .

ACTA BIOMATERIALIA, 2012, 8 (02) :559-569

[7]

Dvir T, 2011, NAT NANOTECHNOL, V6, P720, DOI [10.1038/nnano.2011.160, 10.1038/NNANO.2011.160]

[8] Matrix elasticity directs stem cell lineage specification [J].

Engler, Adam J. ;

Sen, Shamik ;

Sweeney, H. Lee ;

Discher, Dennis E. .

CELL, 2006, 126 (04) :677-689

[9] From the laboratory bench to the patients bedside: An update on clinical trials with mesenchymal stem cells [J].

Giordano, Antonio ;

Galderisi, Umberto ;

Marino, Ignazio R. .

JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 211 (01) :27-35

[10] Combinatorial Polymer Electrospun Matrices Promote Physiologically-Relevant Cardiomyogenic Stem Cell Differentiation [J].

Gupta, Mukesh K. ;

Walthall, Joel M. ;

Venkataraman, Raghav ;

Crowder, Spencer W. ;

Jung, Dae Kwang ;

Yu, Shann S. ;

Feaster, Tromondae K. ;

Wang, Xintong ;

Giorgio, Todd D. ;

Hong, Charles C. ;

Baudenbacher, Franz J. ;

Hatzopoulos, Antonis K. ;

Sung, Hak-Joon .

PLOS ONE, 2011, 6 (12)

← 1 2 3 4 →