Interleukin 28B-related polymorphisms: A pathway for understanding hepatitis C virus infection?

被引:6
作者
Liermann Garcia, Raquel Francine [1 ,2 ]
Moreira, Simone [1 ]
de Araujo Ramos, Ana Lucia [3 ]
Ferreira, Leslie Ecker [1 ]
de Mattos, Angelo Alves [4 ]
Tovo, Cristiane Valle [1 ,4 ]
Nader, Lysandro Alsina [5 ]
Ramos, Juliene Antonio [6 ]
Rondinelli, Edson [6 ]
Dominici, Arnaldo de Jesus [7 ]
Garcia, Christian Evangelista [2 ]
Leite Pinho, Mauro de Souza [1 ]
Brandao-Mello, Carlos Eduardo [8 ]
Villela-Nogueira, Cristiane Alves [3 ]
Condeixa de Franca, Paulo Henrique [1 ]
机构
[1] Univ Regiao Joinville Univille, Dept Med, BR-89219719 Joinville, SC, Brazil
[2] Geral Hos Municipal Sao Jose, Dept Clin Med & Cirurgia, BR-89202000 Joinville, SC, Brazil
[3] Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Fac Med, Dept Clin Med,Serv Hepatol, BR-21941913 Rio De Janeiro, Brazil
[4] Irmandade Santa Casa Misericordia ISCMPA, Serv Gastroenterol & Hepatol, BR-90020090 Porto Alegre, RS, Brazil
[5] Univ Fed Pelotas UFPel, Serv Endoscopia, BR-96010610 Pelotas, RS, Brazil
[6] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21941902 Rio de Janeiro, Brazil
[7] Univ Fed Maranha UFMA, Univ Hosp, Dept Gastroenterol, BR-65020560 Sao Luis, Maranhao, Brazil
[8] Univ Fed Estado Rio de Janeiro, HUGG, Serv Gastroenterol, BR-20270004 Rio de Janeiro, Brazil
关键词
Hepatitis C; Interleukin; 28B; Single nucleotide polymorphisms; Sustained virological response; Brazil; GENETIC-VARIATION; SPONTANEOUS CLEARANCE; VIROLOGICAL RESPONSE; IL28B POLYMORPHISMS; RIBAVIRIN THERAPY; PEGINTERFERON; PREDICTION;
D O I
10.3748/wjg.v19.i42.7399
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To analyze the role of rs12979860 and rs8099917 polymorphisms in hepatitis C virus (HCV) genotype 1 infection of Brazilians. METHODS: A total of 145 adult patients diagnosed with genotype 1 chronic hepatitis C (CHC) who had completed a 48-wk regimen of pegylated-interferon alpha-2a or -2b plus ribavirin combination therapy were recruited from six large urban healthcare centers and 199 healthy blood donors (controls) from a single site between January 2010 and January 2012. Data on the patients' response to treatment was collected. Polymerase chain reaction-restriction fragment length polymorphism genotyping of the interleukin (IL)28B gene fragment encompassing the single nucleotide polymorphisms (SNPs) rs12979860 (C/T) and rs8099917 (T/G) was carried out for 79 of the CHC patients and 199 of the controls. Bi-directional amplicon sequencing of the two SNPs was carried out for the remaining 66 CHC patients. RESULTS: SNP rs12979860 genotyping was successful in 99.5% of the controls and 97.2% of the CHC patients, whereas the SNP rs8099917 genotyping was successful in 95.5% of the controls and 100% of the CHC patients. The genotype and allele distributions for both rs12979860 and rs8099917 were significantly different between the control and CHC patient groups, with significantly higher genotype frequencies of CC and TT in the controls (P = 0.037 and 0.046, respectively) and of TT and GG in the CHC patients (P = 0.0009 and 0.0001, respectively). Analysis of the CHC patients who achieved sustained virological response (SVR) to treatment (n = 55) indicated that the rs12979860 C allele and CC genotype were predictors of SVR (P = 0.02). No significant correlation was found between rs8099917 genotypes and treatment response, but carriers of the T allele showed significantly higher rates of SVR (P = 0.02). Linkage disequilibrium analysis of the group that achieved SVR showed a significant association between rs12979860 and rs8099917 (P = 0.07). CONCLUSION: The higher allele frequency of rs12979860 C and rs8099917 T observed in non-HCV-infected individuals may indicate a potential protective role for these IL28B-related polymorphisms. (C) 2013 Baishideng Publishing Group Co., Limited. All rights reserved.
引用
收藏
页码:7399 / 7404
页数:6
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