Evaluation of Chemical Composition and Antileishmanial and Antituberculosis Activities of Essential Oils of Piper Species

被引：48

作者：

Bernuci, Karine Zanoli ^{[1
]}

Iwanaga, Camila Cristina ^{[1
]}

Mariano Fernandez-Andrade, Carla Maria ^{[1
]}

Lorenzetti, Fabiana Brusco ^{[1
]}

Torres-Santos, Eduardo Caio ^{[2
]}

Faioes, Viviane dos Santos ^{[2
]}

Goncalves, Jose Eduardo ^{[3
,4
,5
]}

do Amaral, Wanderlei ^{[6
]}

Deschamps, Cicero ^{[6
]}

de Lima Scodro, Regiane Bertin ^{[7
]}

Cardoso, Rosilene Fressatti ^{[7
]}

Baldin, Vanessa Pietrowski ^{[7
]}

Garcia Cortez, Diogenes Aparicio ^{[1
,5
]}

机构：

[1] Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, BR-87020900 Maringa, Parana, Brazil

[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Bioquim Tripanosomatideos, BR-21040900 Rio De Janeiro, RJ, Brazil

[3] UniCesumar, Tecnol Limpas, Ave Guerdner 1610, BR-87050390 Maringa, Parana, Brazil

[4] UniCesumar, Promocao Saude, Ave Guerdner 1610, BR-87050390 Maringa, Parana, Brazil

[5] Inst Cesumar Ciencias Tecnol & Inovacao ICETI, Ave Guerdner 1610, BR-87050390 Maringa, Parana, Brazil

[6] Univ Fed Parana, Setor Ciencias Agr, Dept Fitotecnia & Fitossanitarissimo, BR-88035050 Curitiba, Parana, Brazil

[7] Univ Estadual Maringa, Dept Anal Clin & Biomed, BR-87020900 Maringa, Parana, Brazil

来源：

MOLECULES | 2016年 / 21卷 / 12期

关键词：

Piperaceae; GC-MS; essential oil; Leishmania amazonensis; Mycobacterium tuberculosis; ANTIFUNGAL ACTIVITY; IN-VITRO; CLADOSPORIUM-CLADOSPORIOIDES; LEAF EXTRACT; REGNELLII; EUPOMATENOID-5; ANTIOXIDANT; DYSFUNCTION; NEOLIGNANS; LEAVES;

D O I：

10.3390/molecules21121698

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Essential oils from fresh Piperaceae leaves were obtained by hydrodistillation and analyzed by gas chromatography mass spectrometry (GC-MS), and a total of 68 components were identified. Principal components analysis results showed a chemical variability between species, with sesquiterpene compounds predominating in the majority of species analyzed. The composition of the essential oil of Piper mosenii was described for the first time. The cytotoxicity of the essential oils was evaluated in peritoneal macrophages and the oils of P. rivinoides, P. arboretum, and P. aduncum exhibited the highest values, with cytotoxic concentration at 50% (CC50) > 200 mu g/mL. Both P. diospyrifolium and P. aduncum displayed activity against Leishmania amazonensis, and were more selective for the parasite than for the macrophages, with a selectivity index (SI) of 2.35 and > 5.52, respectively. These SI values were greater than the 1 for the standard drug pentamidine. The antileishmanial activity of the essential oils of P. diospyrifolium and P. aduncum was described for the first time. P. rivinoides, P. cernuum, and P. diospyrifolium displayed moderate activity against the Mycobacterium tuberculosis H37Rv bacillus, with a minimum inhibitory concentration (MIC) of 125 mu g/mL. These results are relevant and suggests their potential for therapeutic purposes. Nevertheless, further studies are required to explain the exact mechanism of action of these essential oils.

引用

页数：12

共 44 条

[1] Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase [J].