Structural Composition of Myocardial Infarction Scar in Middle-aged Male and Female Rats: Does Sex Matter?

The present study was designed to determine whether the structural composition of the scar in middle-aged post-myocardial infraction (MI) rats is affected by the biological sex of the animals. A large MI was induced in 12-month-old male (M-MI) and female (F-MI) Sprague-Dawley rats by ligation of the left coronary artery. Four weeks after the MI, rats with transmural infarctions, greater than 50% of the left ventricular (LV) free wall, were evaluated. The extent of LV remodeling and fractional volumes of fibrillar collagen (FC), myofibroblasts, vascular smooth muscle (SM) cells, and surviving cardiac myocytes (CM) in the scars were compared between the two sexes. The left ventricle of post-MI male and female rats underwent a similar degree of remodeling as evidenced by the analogous scar thinning ratio (0.46 +/- 0.02 vs. 0.42 +/- 0.05) and infarct expansion index (1.06 +/- 0.07 vs. 1.12 +/- 0.08), respectively. Most important, the contents of major structural components of the scar revealed no evident difference between M-MI and F-MI rats (interstitial FC, 80.74 +/- 2.08 vs. 82.57 +/- 4.53; myofibroblasts, 9.59 +/- 1.68 vs.9.56 +/- 1.15; vascular SM cells, 2.27 +/- 0.51 vs. 3.38 +/- 0.47; and surviving CM, 3.26 +/- 0.39 vs. 3.05 +/- 0.38, respectively). Our data are the first to demonstrate that biological sex does not influence the structural composition of a mature scar in middle-aged post-MI rats.