Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis

被引：172

作者：

Bomal, Claude ^{[1
]}

Bedon, Frank ^{[1
,2
]}

Caron, Sebastien ^{[1
]}

Mansfield, Shawn D. ^{[4
]}

Levasseur, Caroline ^{[3
]}

Cooke, Janice E. K. ^{[6
]}

Blais, Sylvie ^{[1
]}

Tremblay, Laurence ^{[5
]}

Morency, Marie-Josee

Pavy, Nathalie ^{[1
]}

Grima-Pettenati, Jacqueline ^{[2
]}

Seguin, Armand ^{[3
]}

MacKay, John ^{[1
]}

机构：

[1] Univ Laval, Ctr Etud Foret, Quebec City, PQ G1V 0A6, Canada

[2] CNRS 5546, UMR UPS, F-31326 Castanet Tolosan, France

[3] Laurentian Forestry Ctr, Canadian Forest Serv, Quebec City, PQ G1V 4C7, Canada

[4] Univ British Columbia, Dept Wood Sci, Canada Res Chair Wood & Fibre Qual, Vancouver, BC V6T 1Z4, Canada

[5] Minist Ressources Nat & Faune, DPSP, Quebec City, PQ G1P 3W8, Canada

[6] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2008年 / 59卷 / 14期

关键词：

D O I：

10.1093/jxb/ern234

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The involvement of two R2R3-MYB genes from Pinus taeda L., PtMYB1 and PtMYB8, in phenylpropanoid metabolism and secondary cell wall biogenesis was investigated in planta. These pine MYBs were constitutively overexpressed (OE) in Picea glauca (Moench) Voss, used as a heterologous conifer expression system. Morphological, histological, chemical (lignin and soluble phenols), and transcriptional analyses, i.e. microarray and reverse transcription quantitative PCR (RT-qPCR) were used for extensive phenotyping of MYB-overexpressing spruce plantlets. Upon germination of somatic embryos, root growth was reduced in both transgenics. Enhanced lignin deposition was also a common feature but ectopic secondary cell wall deposition was more strongly associated with PtMYB8-OE. Microarray and RT-qPCR data showed that overexpression of each MYB led to an overlapping up-regulation of many genes encoding phenylpropanoid enzymes involved in lignin monomer synthesis, while misregulation of several cell wall-related genes and other MYB transcription factors was specifically associated with PtMYB8-OE. Together, the results suggest that MYB1 and MYB8 may be part of a conserved transcriptional network involved in secondary cell wall deposition in conifers.

引用

页码：3925 / 3939

页数：15

共 66 条

[1] Efficiency of lignin biosynthesis: a quantitative analysis [J].

Amthor, JS .

ANNALS OF BOTANY, 2003, 91 (06) :673-695

[2] Conifer R2R3-MYB transcription factors:: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca) [J].

Bedon, Frank ;

Grima-Pettenati, Jacqueline ;

Mackay, John .

BMC PLANT BIOLOGY, 2007, 7

[3] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[4]

Bjorkman A., 1956, SVEN PAPPERSTIDN, V59, P477, DOI DOI 10.1016/J.CHROMA.2003.08.046

[5] Lignin biosynthesis [J].

Boerjan, W ;

Ralph, J ;

Baucher, M .

ANNUAL REVIEW OF PLANT BIOLOGY, 2003, 54 :519-546

[6] Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis [J].

Borevitz, JO ;

Xia, YJ ;

Blount, J ;

Dixon, RA ;

Lamb, C .

PLANT CELL, 2000, 12 (12) :2383-2393

[7] Variation in lignin content and composition - Mechanism of control and implications for the genetic improvement of plants [J].

Campbell, MM ;

Sederoff, RR .

PLANT PHYSIOLOGY, 1996, 110 (01) :3-13

[8]

Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468

[9] The MYB transcription factor superfamily of arabidopsis: Expression analysis and phylogenetic comparison with the rice MYB family [J].

Chen, YH ;

Yang, XY ;

He, K ;

Liu, MH ;

Li, JG ;

Gao, ZF ;

Lin, ZQ ;

Zhang, YF ;

Wang, XX ;

Qiu, XM ;

Shen, YP ;

Zhang, L ;

Deng, XH ;

Luo, JC ;

Deng, XW ;

Chen, ZL ;

Gu, HY ;

Qu, LJ .

PLANT MOLECULAR BIOLOGY, 2006, 60 (01) :107-124

[10] MAIZE POLYUBIQUITIN GENES - STRUCTURE, THERMAL PERTURBATION OF EXPRESSION AND TRANSCRIPT SPLICING, AND PROMOTER ACTIVITY FOLLOWING TRANSFER TO PROTOPLASTS BY ELECTROPORATION [J].

CHRISTENSEN, AH ;

SHARROCK, RA ;

QUAIL, PH .

PLANT MOLECULAR BIOLOGY, 1992, 18 (04) :675-689

← 1 2 3 4 5 6 7 →