The response of LuxS/AI-2 quorum sensing in Lactobacillus fermentum 2-1 to changes in environmental growth conditions

被引：44

作者：

Gu, Yue ^{[1
]}

Li, Bo ^{[1
]}

Tian, Jianjun ^{[1
]}

Wu, Rong ^{[1
]}

He, Yinfeng ^{[1
]}

机构：

[1] Inner Mongolia Agr Univ, Coll Food Sci & Engn, 306 Zhaowuda Rd, Hohhot 010018, Inner Mongolia, Peoples R China

来源：

ANNALS OF MICROBIOLOGY | 2018年 / 68卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Quorum sensing; Lactobacillus fermentum 2-1; Autoinducer-2; Transcription; Environmental stresses; LACTIC-ACID BACTERIA; DEINOCOCCUS-RADIODURANS; KLEBSIELLA-PNEUMONIAE; BIOFILM FORMATION; ESCHERICHIA-COLI; OXIDATIVE STRESS; RHAMNOSUS GG; LUXS; AUTOINDUCER-2; METABOLISM;

D O I：

10.1007/s13213-018-1337-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Quorum sensing (QS) is a communication mechanism based on the production of autoinducers in order to regulate cooperative behavior and physiological traits among bacteria. Autoinducer-2 (AI-2) is mediated by luxS and pfs genes and is used for both intra- and interspecies communication. The effects of environmental stresses, such as pH, temperature, osmotic, and nutrient stresses, were investigated on the LuxS/AI-2 QS system in Lactobacillus fermentum 2-1. The AI-2 activity in L. fermentum 2-1 was induced by stress responses to acid shock, high temperature, and nutrient inadequacy while a decrease in activity was correlated with high pH, low temperature, and osmotic stress. Maximum transcription of luxS and pfs was achieved after L. fermentum 2-1 was exposed to acid shock, low temperature, high concentration of NaCl, and diluted MRS broth. The results indicate that various stresses affected the LuxS/AI-2 QS system, which may be involved in the resistance process of the L. fermentum 2-1.

引用

页码：287 / 294

页数：8

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