IDENTIFICATION OF BUFFALO (Bubalus bubalis) MEAT USING PCR TARGETING MITOCHONDRIAL D-LOOP GENE

被引：0

作者：

Kumar, Deepak ^{[1
]}

Singh, S. P. ^{[1
]}

Umapathi, V. ^{[1
]}

Nagappa, K. ^{[1
]}

Gaurav, Abhishek ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Vet Publ Hlth, Bareilly, Uttar Pradesh, India

来源：

BUFFALO BULLETIN | 2011年 / 30卷 / 03期

关键词：

meat; adulteration; buffalo; d-loop; PCR; POLYMERASE-CHAIN-REACTION; SPECIES IDENTIFICATION; PORK; PRODUCTS;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A novel polymerase chain reaction (PCR) was developed for the identification of buffalo (Bubalus bubalis) meat using newly designed primers targeting the mitochondrial D-loop region. Buffalo-specific primers were designed against a conserved region of mitochondrial d-loop that amplified buffalo specific region of 358 bp in size. The specificity of primers was confirmed by PCR analysis of DNA from related domestic animal meats i.e. cattle, goat, sheep, pig and chicken. The PCR assay was checked for repeatability using DNA isolated from different buffalo meat samples and was validated. Buffalo species-specific PCR developed in this study presents a means of identification of buffalo meat as a reliable tool to avoid the fraudulent substitution and adulteration of buffalo meat.

引用

页码：170 / 176

页数：7

共 17 条

[11] Complete nucleotide sequences of the domestic cat (Felis catus) mitochondrial genome and a transposed mtDNA tandem repeat (Numt) in the nuclear genome [J].

Lopez, JV ;

Cevario, S ;

OBrien, SJ .

GENOMICS, 1996, 33 (02) :229-246

[12]

Malisa AL, 2006, AFR J BIOTECHNOL, V5, P1588

[13] Development of a polymerase chain reaction assay for species identification of goose and mule duck in foie gras products [J].

Rodríguez, MA ;

García, T ;

González, I ;

Asensio, L ;

Mayoral, B ;

López-Calleja, I ;

Hernández, PE ;

Martín, R .

MEAT SCIENCE, 2003, 65 (04) :1257-1263

[14] Methodology and development of prediction equations for the determination of pork substitution in veal [J].

Toorop, RM ;

Murch, SJ ;

Ball, RO .

FOOD RESEARCH INTERNATIONAL, 1997, 30 (08) :629-636

[15] Capillary electrophoresis for the analysis of meat authenticity [J].

Vallejo-Cordoba, B ;

González-Córdova, AF ;

Mazorra-Manzano, MA ;

Rodríguez-Ramírez, R .

JOURNAL OF SEPARATION SCIENCE, 2005, 28 (9-10) :826-836

[16] Novel universal primers establish identity of an enormous number of animal species for forensic application [J].

Verma, SK ;

Singh, L .

MOLECULAR ECOLOGY NOTES, 2003, 3 (01) :28-31

[17] Establishment and application of a polymerase chain reaction for the identification of beef [J].

Zhang, GL ;

Zheng, MG ;

Zhou, ZJ ;

Ouyang, HS ;

Lu, Q .

MEAT SCIENCE, 1999, 51 (03) :233-236

← 1 2 →