Effects of low-intensity pulsed ultrasound on proliferation of bone mesenchymal stem cells

被引：0

作者：

Yu Haisheng ^{[1
]}

Wang Yan ^{[1
]}

Hu Shuai ^{[1
]}

Liu Zheng ^{[1
]}

Chen Wenzhi ^{[2
]}

机构：

[1] Chongqing Med Univ, State Key Lab Ultrasound Engn Med, Minist Sci & Technol, Chongqing Key Lab Ultrasound Med & Engn,Dept Biom, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 2, Clin Ctr Tumor Therapy, Chongqing 400010, Peoples R China

来源：

ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 2 | 2011年

关键词：

Tissue engineering; Bone mesenchaymal stein cells; Low-intensity pulsed ultrasound; Cell proliferation;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Objective: To explore the feasibility of promoting bone mesenchymal stem cells (BMSCs) proliferation by low intensity pulsed ultrasound(LIPUS). Methods: The third generation of BMSCs was seeded in 6-well plates and randomly divided into 2 groups: LIPUS groups and blank-control groups. Growth of cells was observed daily under light microscope and the cell proliferation was determined by MTT at 0 day pre-irradiation and 1, 3, 5day post-irradiation; Cell cycle changes were analyzed at 0 day pre-irradiation and 1, 3, 5, 7day post-irradiation using the flow cytometry. Results: Cell proliferation of the LIPUS group was significantly more active than that of the control-group. LIPUS group also had more intensive cell colonies; MTT test showed that cell proliferation in the LIPUS group of 1d, 3d and 5d post-irradiation were significantly higher than that in the control group (P<0.05); Cell cycle changes tested by flow cytometry showed that the cell proliferation rate (S+G2%) of the LIPUS-group was significantly higher than that of the control group after irradiation (P<0.01).Conclusion: LIPUS could promote the proliferation of BMSCs and motivate quiescent state BMSCs to enter division state.

引用

页码：373 / 378

页数：6

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