Antiviral and antiproliferative effects of canine interferon-λ1

被引：9

作者：

Ichihashi, Tomonori ^{[1
]}

Asano, Atsushi ^{[1
]}

Usui, Tatsufumi ^{[2
,4
]}

Takeuchi, Takashi ^{[3
]}

Watanabe, Yasuko ^{[5
]}

Yamano, Yoshiaki ^{[1
]}

机构：

[1] Tottori Univ, Vet Biochem Lab, Tottori 6808553, Japan

[2] Tottori Univ, Lab Vet Hyg, Tottori 6808553, Japan

[3] Tottori Univ, Sch Vet Med, Lab Lab Anim Sci, Tottori 6808553, Japan

[4] Tottori Univ, Avian Zoonosis Res Ctr, Fac Agr, Tottori 6808553, Japan

[5] Tottori Prefectural Inst Publ Hlth & Environm Sci, Tottori 6820704, Japan

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2013年 / 156卷 / 1-2期

关键词：

Antiviral activity; Antiproliferation; Dog; Interferon-stimulated genes; IFN-lambda; 1; IFN-LAMBDA; III INTERFERON; ANTITUMOR-ACTIVITY; EXPRESSION; CELLS; ALPHA; IL-29;

D O I：

10.1016/j.vetimm.2013.09.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interferon (IFN)-lambda s, members of the type III IFN group, were recently identified in several vertebrates. Although IFN-lambda s have the potential to be utilized as antiviral and antitumor agents in veterinary medicine, the biological properties of IFN-lambda s have not yet been studied in companion animals. In this study, we analyzed the expression of canine IFN-lambda s and their receptors, produced a recombinant canine IFN-lambda 1 protein, and investigated its antiviral and antiproliferative activities using a canine kidney epithelial cell line, MDCK cells. MDCK cells were found to express type III IFN molecules, IFN-lambda 1 and IFN-lambda 3, and the receptors, IFN lambda R1 and IL10R2. IFN-lambda 1 was induced faster than IFN-lambda 3 by stimulation with poly (I:C). His-tagged IFN-lambda 1 protein expressed in Escherichia coli inhibited cytolytic plaque formation by influenza A virus infection, and induced the expression of interferon-stimulated genes, Mx1 and OAS1, in MDCK cells. In addition, recombinant IFN-lambda 1 inhibited the proliferation of MDCK cells slightly. These effects were observed in a dose-dependent manner. These results indicate that canine IFN-lambda 1 has antiviral effect, and suggest the potential applicability of canine IFN-lambda 1 as a therapeutic agent. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：141 / 146

页数：6

共 29 条

[1] Lambda interferon (IFN-λ), a type III IFN, is induced by viruses and IFNs and displays potent antiviral activity against select virus infections in vivo [J].

Ank, N ;

West, H ;

Bartholdy, C ;

Eriksson, K ;

Thomsen, AR ;

Paludan, SR .

JOURNAL OF VIROLOGY, 2006, 80 (09) :4501-4509

[2] An important role for type III interferon (IFN-λ/IL-28) in TLR-induced antiviral activity [J].

Ank, Nina ;

Iversen, Marie B. ;

Bartholdy, Christina ;

Staeheli, Peter ;

Hartmann, Rune ;

Jensen, Uffe B. ;

Dagnaes-Hansen, Frederik ;

Thomsen, Allan R. ;

Chen, Zhi ;

Haugen, Harald ;

Klucher, Kevin ;

Paludan, Soren R. .

JOURNAL OF IMMUNOLOGY, 2008, 180 (04) :2474-2485

[3] Enhanced circulating half-life and antitumor activity of a site-specific pegylated interferon-α protein therapeutic [J].

Bell, Stacie J. ;

Fam, Christine M. ;

Chlipala, Elizabeth A. ;

Carlson, Sharon J. ;

Lee, Ji I. ;

Rosendahl, Mary S. ;

Doherty, Daniel H. ;

Cox, George N. .

BIOCONJUGATE CHEMISTRY, 2008, 19 (01) :299-305

[4] IL-28A and IL-29 mediate antiproliferative and antiviral signals in intestinal epithelial cells and murine CMV infection increases colonic IL-28A expression [J].

Brand, S ;

Beigel, F ;

Olszak, T ;

Zitzmann, K ;

Eichhorst, ST ;

Otte, JM ;

Diebold, J ;

Diepolder, H ;

Adler, B ;

Auernhammer, CJ ;

Göke, B ;

Dambacher, J .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2005, 289 (05) :G960-G968

[5] Interferon-Lambda: A New Addition to an Old Family [J].

Donnelly, Raymond P. ;

Kotenko, Sergei V. .

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 2010, 30 (08) :555-564

[6] Pharmacology and therapeutic potential of interferons [J].

George, Peter M. ;

Badiger, Rekha ;

Alazawi, William ;

Foster, Graham R. ;

Mitchell, Jane A. .

PHARMACOLOGY & THERAPEUTICS, 2012, 135 (01) :44-53

[7] Dynamin-like MxA GTPase: Structural Insights into Oligomerization and Implications for Antiviral Activity [J].

Haller, Otto ;

Gao, Song ;

von der Malsburg, Alexander ;

Daumke, Oliver ;

Kochs, Georg .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (37) :28419-28424

[8] IFN-λs [J].

Kotenko, Sergei V. .

CURRENT OPINION IN IMMUNOLOGY, 2011, 23 (05) :583-590

[9] IFN-λs mediate antiviral protection through a distinct class II cytokine receptor complex [J].

Kotenko, SV ;

Gallagher, G ;

Baurin, VV ;

Lewis-Antes, A ;

Shen, ML ;

Shah, NK ;

Langer, JA ;

Sheikh, F ;

Dickensheets, H ;

Donnelly, RP .

NATURE IMMUNOLOGY, 2003, 4 (01) :69-77

[10] Interferon-λ induces G1 phase arrest or apoptosis in oesophageal carcinoma cells and produces anti-tumour effects in combination with anti-cancer agents [J].

La, Quanhai ;

Kawamura, Kiyoko ;

Ma, Guangyu ;

Iwata, Fumi ;

Numasaki, Muneo ;

Suzuki, Nobuo ;

Shimada, Hideaki ;

Tagawa, Masatoshi .

EUROPEAN JOURNAL OF CANCER, 2010, 46 (01) :180-190

← 1 2 3 →