A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019

被引：105

作者：

Cai, Xue-fei ^{[1
]}

Chen, Juan ^{[1
]}

Hu, Jie-li ^{[1
]}

Long, Quan-xin ^{[1
]}

Deng, Hai-jun ^{[1
]}

Liu, Ping ^{[2
]}

Fan, Kai ^{[3
]}

Liao, Pu ^{[4
]}

Liu, Bei-zhong ^{[5
]}

Wu, Gui-cheng ^{[6
]}

Chen, Yao-kai ^{[7
]}

Li, Zhi-jie ^{[4
]}

Wang, Kun ^{[4
]}

Zhang, Xiao-li ^{[5
]}

Tian, Wen-guang ^{[5
]}

Xiang, Jiang-lin ^{[6
]}

Du, Hong-xin ^{[6
]}

Wang, Jing ^{[7
]}

Hu, Yuan ^{[1
]}

Tang, Ni ^{[1
]}

Lin, Yong ^{[1
]}

Ren, Ji-hua ^{[1
]}

Huang, Lu-yi ^{[1
]}

Wei, Jie ^{[1
]}

Gan, Chun-yang ^{[1
]}

Chen, Yan-meng ^{[1
]}

Gao, Qing-zhu ^{[1
]}

Chen, A-mei ^{[1
]}

He, Chang-long ^{[1
]}

Wang, Dao-Xin ^{[8
]}

Hu, Peng ^{[1
]}

Zhou, Fa-Chun ^{[9
]}

Huang, Ai-long ^{[1
]}

Wang, De-qiang ^{[1
,10
]}

机构：

[1] Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Room 613,Coll Life Sci Bldg,1 YiXueYuan Rd, Chongqing 400016, Peoples R China

[2] BioSci Co LTD, Tianjin, Peoples R China

[3] PEG Bio Biopharm Co LTD, Chongqing, Peoples R China

[4] Chongqing Peoples Hosp, Lab Dept, Chongqing, Peoples R China

[5] Chongqing Med Univ, Yangchuan Hosp, Chongqing, Peoples R China

[6] Chongqing Three Gorges Cent Hosp, Chongqing, Peoples R China

[7] Publ Hlth Ctr, Chongqing, Peoples R China

[8] Chongqing Med Univ, Affiliated Hosp 2, Dept Respirat, Chongqing, Peoples R China

[9] Chongqing Med Univ, Affiliated Hosp 1, Dept Emergency, Chongqing, Peoples R China

[10] Chongqing Med Univ, Coll Lab Med, Chongqing, Peoples R China

来源：

JOURNAL OF INFECTIOUS DISEASES | 2020年 / 222卷 / 02期

关键词：

chemiluminescence immunoassay; COVID-19; SARS-CoV-2; serological test;

D O I：

10.1093/infdis/jiaa243

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel beta-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. Methods. In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SA RS-CoV-2. Results. To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. Conclusions. Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.

引用

页码：189 / 193

页数：5

共 5 条

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[4] [Anonymous], 2016, CLIN INFECT DIS, DOI DOI 10.1093/CID/CIV951
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