Dynamic and Reversible Fluorescence Imaging of Superoxide Anion Fluctuations in Live Cells and in Vivo

被引：208

作者：

Zhang, Wen ^{[1
]}

Li, Ping ^{[1
]}

Yang, Fan ^{[1
]}

Hu, Xiufen ^{[1
]}

Sun, Chuanzhi ^{[1
]}

Zhang, Wei ^{[1
]}

Chen, Dezhan ^{[1
]}

Tang, Bo ^{[1
]}

机构：

[1] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Engn Res Ctr Pesticide & Med Intermediate Clean P, Key Lab Mol & Nano Probes,Minist Educ, Jinan 250014, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 40期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

ISCHEMIA-REPERFUSION; PROBE; RADICALS; STRESS; MICROSCOPY; REACTIVITY;

D O I：

10.1021/ja408524j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O-2(center dot-)) is the precursor of other ROS, exploring O-2(center dot-). fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O-2(center dot-) fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O-2(center dot-) under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O-2(center dot-). concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O-2(center dot-). in live cells and in vivo.

引用

页码：14956 / 14959

页数：4

共 24 条

[1] Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds [J].