Wide Dissemination of GES-Type Carbapenemases in Acinetobacter baumannii Isolates in Kuwait

被引：66
作者：
Bonnin, Remy A. [1 ,3 ]
Rotimi, Vincent O. [2 ]
Al Hubail, Mona [2 ]
Gasiorowski, Elise [1 ,3 ]
Al Sweih, Noura [2 ]
Nordmann, Patrice [1 ,3 ]
Poirel, Laurent [1 ,3 ]
机构：
[1] Hop Bicetre, AP HP, INSERM, Serv Bacteriol Virol,Fac Med,U914, Le Kremlin Bicetre, France
[2] Kuwait Univ, Dept Microbiol, Fac Med, Safat, Kuwait
[3] Univ Paris Sud, F-94275 Le Kremlin Bicetre, France
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2013年 / 57卷 / 01期
关键词：
SPECTRUM BETA-LACTAMASES; GENETIC-BASIS; PCR; RESISTANCE; MULTICENTER; DIVERSITY; DNA;
D O I：
10.1128/AAC.01384-12
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Acinetobacter baumannii is an opportunistic pathogen that is an important source of nosocomial infections. Production of extended-spectrum beta-lactamases (ESBLs) of the GES type in A. baumannii has been increasingly reported, and some of these GES-type enzymes possess some carbapenemase activity. Our aim was to analyze the resistance determinants and the clonal relationships of carbapenem-nonsusceptible A. baumannii clinical isolates recovered from hospitals in Kuwait. A total of 63 isolates were analyzed, and all were found to be positive for blaGES-type genes. One isolate harbored the bla(GES-14) gene encoding an ESBL with significant carbapenemase activity, whereas the other isolates harbored the bla(GES-11) ESBL gene. Thirty-three isolates coharbored the bla(OXA-23) and bla(GES-11) genes. Analyses of the genetic locations indicated that the bla(GES-11/-14) genes were plasmid located. It is noteworthy that the bla(OXA-23) and bla(GES-11) genes were colocated onto a single plasmid. Nine different pulsotypes were observed among the 63 isolates. This study showed the emergence of GES-type ESBLs in A. baumannii in Kuwait, further suggesting that the Middle East region might be a reservoir for carbapenemase-producing A. baumannii.
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页码：183 / 188
页数：6
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