Characterisation of phenolics by LC-UV/Vis, LC-MS/MS and sugars by GC in Melicoccus bijugatus Jacq. 'Montgomery' fruits

被引：120

作者：

Bystrom, Laura M. ^{[3
]}

Lewis, Betty A. ^{[3
]}

Brown, Dan L. ^{[2
]}

Rodriguez, Eloy ^{[4
]}

Obendorf, Ralph L. ^{[1
]}

机构：

[1] Cornell Univ, Dept Crop & Soil Sci, Ithaca, NY 14850 USA

[2] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA

[3] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA

[4] Cornell Univ, Dept Plant Biol, Ithaca, NY USA

来源：

FOOD CHEMISTRY | 2008年 / 111卷 / 04期

关键词：

Melicoccus bijugatus; fruit phenolics; fruit sugars; HPLC; LC-MS/MS;

D O I：

10.1016/j.foodchem.2008.04.058

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Fruits of the native South American tree Melicoccus bijugatus Jacq. (Sapindaceae) are consumed for both dietary and medicinal purposes, but limited information is available about the phytochemistry and health Value of M. bijugatus fruits. Fruit tissues of the Florida Montgomery cultivar were assessed for sugars, using gas chromatography, and for total phenolics, using UV spectroscopy. Reverse-phase high performance liquid chromatography (HPLC) fingerprints of crude methanolic Pulp, embryo and seed coat extracts were obtained at 280 nm. Phenolics were characterised by both HPLC-UV/Vis analysis and HPLC electrospray ionisation tandem mass spectrometry. Major sugars detected in the pulp and embryo extracts were Sucrose, followed by glucose and fructose. The glucose: fructose ratio was 1:1 in the pulp and 0.1 : I in the embryo. Total phenolic concentrations of the fruit tissues were in the order: seed coat > embryo > pulp. Phenolic acids were identified mostly in pulp tissues. Phenolic acids, flavonoids, procyanidins and catechins were identified in embryo tissues, and higher molecular weight procyanidins were identified in seed coat tissues. This Study provides new information about the phytochemistry and the potential health value of the Montgomery cultivar M. bijugatus fruit tissues. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1017 / 1024

页数：8

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