When to harvest peripheral-blood stem cells after mobilization therapy: Prediction of CD34-positive cell yield by preceding day CD34-positive concentration in peripheral blood

Purpose: To evaluate whether CD34(+) yield form a single peripheral-blood stem-cell (PBSC) harvest could To evaluate whether the CD34+ yield from predicted by measurement of the patient's circulating WBC and CD34+ cell concentrations on the day before harvest. Patients and Methods: Thirty-nine patients with hematologic or nonhematologic malignancy underwent 41 stem-cell mobilization episodes with cytotoxic chemotherapy and/or granulocyte colony-stimulating factor (G-CSF), and a total of 63 leukapheresis procedures were performed. Peripheral-blood samples were analyzed for WBC and CD34+ cell concentrations both on the day before and the day of leukapheresis. Results: The median WBC and CD34(+) concentrations on the day preceding leukapheresis were 10.0 x 10(9)/L (range, 0.4 to 44.4) and 24.9 x 10(6)/L (range, 0.1 to 349.4), respectively. On the day of harvest, the corresponding figures were 15.1 x 10(9)/L(range, 1.5 to 52.6) and 29.3 x 10(6)/L(range, 0.1 to 543.1), respectively. The median CD34(+) cell number collected in a single leukapheresis was 2.6 x 10(6)/kg body weight (range, 0.1 to 26.1). Both the preceding day (r =.84, P <.001) and harvest day (r =.95, P <.001) CD34(+) circulating concentrations correlated significantly with the number of CD34(+) cells per kilogram collected at leukapheresis. The correlation between CD34(+) cells per kilogram collected and harvest day WBC count was also significant (r =.43, P <.001), but with the preceding day WBC count was nonsignificant (r =.15, P >.2). Conclusion: The number of CD34(+) cells harvested in a single leukapheresis can be predicted by measurement of the preceding day peripheral-blood circulating CD34(+) concentration, and on the basis of these data a table of probable CD34+ cell yield has been constructed. This correlation may facilitate the efficient organization of leukapheresis procedures. (C) 1996 by American Society of Clinical Oncology.