The adverse effects of phoxim exposure in the midgut of silkworm, Bombyx mori

被引：51

作者：

Gu, ZhiYa ^{[1
]}

Zhou, YiJun ^{[1
]}

Xie, Yi ^{[1
]}

Li, FanChi ^{[1
]}

Ma, Lie ^{[1
]}

Sun, ShanShan ^{[1
]}

Wu, Yu ^{[1
]}

Wang, BinBin ^{[1
]}

Wang, JuMei ^{[1
]}

Hong, Fashui ^{[1
]}

Shen, WeiDe ^{[1
,2
]}

Li, Bing ^{[1
,2
]}

机构：

[1] Soochow Univ, Sch Basic Med & Biol Sci, Suzhou 215123, Jiangsu, Peoples R China

[2] Soochow Univ, Natl Engn Lab Modern Silk, Suzhou 215123, Jiangsu, Peoples R China

来源：

CHEMOSPHERE | 2014年 / 96卷

基金：

国家教育部博士点专项基金资助; 国家高技术研究发展计划(863计划);

关键词：

Bombyx mori; Phoxim insecticide; Detoxification; Apoptosis; Immune defense; Digital gene expression; ACETYLCHOLINESTERASE GENE; DOMESTICATED SILKWORM; IMMUNE-RESPONSE; EXPRESSION; DROSOPHILA; PATHWAYS; TOLL; RESISTANCE; REVEALS; TRANSCRIPTOME;

D O I：

10.1016/j.chemosphere.2013.06.089

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The silkworm is an important economic insect. Poisoning of silkworms by organophosphate pesticides causes tremendous loss to the sericulture. In this study, Solexa sequencing technology was performed to profile the gene expression changes in the midgut of silkworms in response to 24 h of phoxim exposure and the impact on detoxification, apoptosis and immune defense were addressed. The results showed that 254 genes displayed at least 2.0-fold changes in expression levels, with 148 genes up-regulated and 106 genes down-regulated. Cytochrome P450 played an important role in detoxification. Histopathology examination and transmission electron microscope revealed swollen mitochondria and disappearance of the cristae of mitochondria, which are the important features in insect apoptotic cells. Cytochrome C release from mitochondria into the cytoplasm was confirmed. In addition, the Toll and immune deficiency (IMD) signal pathways were all inhibited using qRT-PCR. Our results could help better understand the impact of phoxim exposure on silkworm. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：33 / 38

页数：6

共 43 条

[1] Toll signaling pathways in the innate immune response [J].

Anderson, KV .

CURRENT OPINION IN IMMUNOLOGY, 2000, 12 (01) :13-19

[2] The significance of digital gene expression profiles [J].

Audic, S ;

Claverie, JM .

GENOME RESEARCH, 1997, 7 (10) :986-995

[3] Toward the $1000 human genome [J].

Bennett, ST ;

Barnes, C ;

Cox, A ;

Davies, L ;

Brown, C .

PHARMACOGENOMICS, 2005, 6 (04) :373-382

[4] Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells [J].

Boonstra, J ;

Post, JA .

GENE, 2004, 337 :1-13

[5] Organophosphorus compound-induced modification of SH-SY5Y human neuroblastoma mitochondrial transmembrane potential [J].

Carlson, K ;

Ehrich, M .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1999, 160 (01) :33-42

[6] Organophosphorus compound-induced apoptosis in SH-SY5Y human neuroblastoma cells [J].

Carlson, K ;

Jortner, BS ;

Ehrich, M .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2000, 168 (02) :102-113

[7] Characterization of an immunodeficiency mutant in Drosophila [J].

Corbo, JC ;

Levine, M .

MECHANISMS OF DEVELOPMENT, 1996, 55 (02) :211-220

[8] Origins of immunity: Relish, a compound Rel-like gene in the antibacterial defense of Drosophila [J].

Dushay, MS ;

Asling, B ;

Hultmark, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (19) :10343-10347

[9] A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the toll pathway [J].

Ferrandon, D ;

Jung, AC ;

Criqui, MC ;

Lemaitre, B ;

Uttenweiler-Joseph, S ;

Michaut, L ;

Reichhart, JM ;

Hoffmann, JA .

EMBO JOURNAL, 1998, 17 (05) :1217-1227

[10] MODIFICATION OF ACETYLCHOLINESTERASE AS A MECHANISM OF RESISTANCE EO INSECTICIDES [J].

FOURNIER, D ;

MUTERO, A .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1994, 108 (01) :19-31

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