Variants of U1 small nuclear RNA assemble into spliceosomal complexes

被引：5

作者：

Pereira-Simon, S ^{[1
]}

Sierra-Montes, JM ^{[1
]}

Ayesh, K ^{[1
]}

Martinez, L ^{[1
]}

Socorro, A ^{[1
]}

Herrera, RJ ^{[1
]}

机构：

[1] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA

来源：

INSECT MOLECULAR BIOLOGY | 2004年 / 13卷 / 02期

关键词：

U1 snRNA variants; spliceosomal assembly; Bombyx mori; RNA secondary structure; free energy values; phylogenetic analyses;

D O I：

10.1111/j.0962-1075.2004.00476.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, the existence of additional U1 snRNA variants in the posterior silk gland of the Bombyx mori Nistari strain from India was investigated. Three new U1 variants were detected. One of the new isoforms (U1 SG1) was found to be preferentially assembled into high molecular weight spliceosomal complexes in comparison with the total cellular lysate RNA control. Structural and nucleotide differences were examined in these new isoforms and compared with the previously reported U1 variants. Free energy (DeltaG) values for the entire U1 snRNA secondary structures as well as the individual stem/loops (I, II, III and IV) domains of the isoforms were estimated to determine their structural stability.

引用

页码：189 / 194

页数：6

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