Engineered Escherichia coli for simultaneous utilization of galactose and glucose

被引：25

作者：

Lim, Hyun Gyu ^{[1
]}

Seo, Sang Woo ^{[1
]}

Jung, Gyoo Yeol ^{[1
,2
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Chem Engn, Pohang 790784, Gyeongbuk, South Korea

[2] Pohang Univ Sci & Technol, Sch Interdisciplinary Biosci & Bioengn, Pohang 790784, Gyeongbuk, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 135卷

基金：

新加坡国家研究基金会;

关键词：

Galactose; Pathway engineering; Synthetic biology; Metabolic engineering; GENE; REPRESSION; TRANSCRIPTION; EXPRESSION; SYSTEM; OPERON;

D O I：

10.1016/j.biortech.2012.10.124

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study, the Leloir pathway of galactose metabolism was rebuilt in Escherichia coli to remove CCR and amplify galactose utilization rate. All genes encoding pathway enzymes were expressed under the control of a synthetic module that included promoters, 5'-untranslated regions, and terminators as a re-organized single operon in the chromosome. The engineered strain showed both an enhanced galactose utilization rate and the capacity to simultaneously assimilate galactose and glucose. This work demonstrates the feasibility of using synthetic biology tools to re-build biological systems for engineering purpose. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：564 / 567

页数：4

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