New paradigm for macromolecular crystallography experiments at SSRL: automated crystal screening and remote data collection

被引：112

作者：

Soltis, S. Michael ^{[1
]}

Cohen, Aina E. ^{[1
]}

Deacon, Ashley ^{[1
]}

Eriksson, Thomas ^{[1
]}

Gonzalez, Ana ^{[1
]}

McPhillips, Scott ^{[1
]}

Chui, Hsui ^{[1
]}

Dunten, Pete ^{[1
]}

Hollenbeck, Michael ^{[1
]}

Mathews, Irimpan ^{[1
]}

Miller, Mitch ^{[1
]}

Moorhead, Penjit ^{[1
]}

Phizackerley, R. Paul ^{[1
]}

Smith, Clyde ^{[1
]}

Song, Jinhu ^{[1
]}

van dem Bedem, Henry ^{[1
]}

Ellis, Paul ^{[1
]}

Kuhn, Peter ^{[1
]}

McPhillips, Timothy ^{[1
]}

Sauter, Nicholas ^{[1
]}

Sharp, Kenneth ^{[1
]}

Tsyba, Irina ^{[1
]}

Wolf, Guenter ^{[1
]}

机构：

[1] SLAC, SSRL, Menlo Pk, CA 95124 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2008年 / 64卷

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1107/S0907444908030564

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Complete automation of the macromolecular crystallography experiment has been achieved at SSRL through the combination of robust mechanized experimental hardware and a flexible control system with an intuitive user interface. These highly reliable systems have enabled crystallography experiments to be carried out from the researchers' home institutions and other remote locations while retaining complete control over even the most challenging systems. A breakthrough component of the system, the Stanford Auto-Mounter (SAM), has enabled the efficient mounting of cryocooled samples without human intervention. Taking advantage of this automation, researchers have successfully screened more than 200 000 samples to select the crystals with the best diffraction quality for data collection as well as to determine optimal crystallization and cryocooling conditions. These systems, which have been deployed on all SSRL macromolecular crystallography beamlines and several beamlines worldwide, are used by more than 80 research groups in remote locations, establishing a new paradigm for macromolecular crystallography experimentation.

引用

页码：1210 / 1221

页数：12

共 35 条

[1] Structural basis of transcription: An RNA polymerase II-TFIIB cocrystal at 4.5 angstroms [J].