Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

被引：24

作者：

Heng, NCK

Jenkinson, HF

Tannock, GW

机构：

[1] UNIV OTAGO,DEPT MICROBIOL,DUNEDIN,NEW ZEALAND

[2] UNIV OTAGO,DEPT ORAL BIOL,DUNEDIN,NEW ZEALAND

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 08期

关键词：

D O I：

10.1128/AEM.63.8.3336-3340.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.

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收藏

页码：3336 / 3340

页数：5

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