Transcriptome analysis of three cultivars of Poria cocos reveals genes related to the biosynthesis of polysaccharides

被引：15
|
作者：
Yang, Lan [1 ,2 ]
Tang, Juan [1 ,2 ]
Chen, Jin-Jun [1 ]
Peng, Ai-Yun [3 ]
Wang, Qi-Ming [1 ,2 ]
Rao, Li-Qun [1 ,2 ]
Yang, Hua [1 ,2 ]
Zhang, Xian-Wen [1 ,2 ]
Yang, Huan-Zhi [1 ,2 ]
Zhang, Chen [1 ,2 ]
Peng, Guo-Ping [1 ,2 ]
机构：
[1] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China
[2] Hunan Engn Lab Good Agr Practice & Comprehens Uti, Changsha 410128, Hunan, Peoples R China
[3] Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Guangdong, Peoples R China
来源：
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH | 2019年 / 21卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Poria cocos; polysaccharides; polysaccharides biosynthesis; transcriptome; qRT-PCR; BACTERIAL;
D O I：
10.1080/10286020.2018.1494159
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Poria cocos (P. cocos) polysaccharides (PCPs) are used to improve immunity and possess antitumor activities. We compared three cultivars of P. cocos (5.78, XJ 28 and JHYH) PCP contents. Then we determined that malZ, galA, SORD, gnl and bglX are key enzymes within the PCP biosynthetic pathway by using HiSeq2500 transcriptome and qRT-PCR validation. Our results provide more detailed information about the PCP biosynthesis pathway at the molecular level in P. cocos and establish the functions for the molecular breeding to produce polysaccharides in general for therapeutic use in Chinese medicinal plants. [GRAPHICS] .
引用
收藏
页码：462 / 475
页数：14
相关论文
共 50 条
  • [21] Transcriptome analysis reveals novel genes involved in anthocyanin biosynthesis in the flesh of peach
    Cao, Ke
    Ding, Tiyu
    Mao, Dongmin
    Zhu, Gengrui
    Fang, Weichao
    Chen, Changwen
    Wang, Xinwei
    Wang, Lirong
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2018, 123 : 94 - 102
  • [22] Transcriptome Analysis Reveals Putative Genes Involved in Iridoid Biosynthesis in Rehmannia glutinosa
    Sun, Peng
    Song, Shuhui
    Zhou, Lili
    Zhang, Bing
    Qi, Jianjun
    Li, Xianen
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (10): : 13748 - 13763
  • [23] Transcriptome analysis of Asparagus officinalis reveals genes involved in the biosynthesis of rutin and protodioscin
    Yi, Tae Gyu
    Yeoung, Young Rog
    Choi, Ik-Young
    Park, Nam-Il
    PLOS ONE, 2019, 14 (07):
  • [24] Transcriptome analysis and identification of genes related to terpenoid biosynthesis in Cinnamomum camphora
    Caihui Chen
    Yongjie Zheng
    Yongda Zhong
    Yangfang Wu
    Zhiting Li
    Li-An Xu
    Meng Xu
    BMC Genomics, 19
  • [25] Transcriptome analysis and identification of genes related to terpenoid biosynthesis in Cinnamomum camphora
    Chen, Caihui
    Zheng, Yongjie
    Zhong, Yongda
    Wu, Yangfang
    Li, Zhiting
    Xu, Li-An
    Xu, Meng
    BMC GENOMICS, 2018, 19
  • [26] Poria cocos polysaccharides alleviate cyclophosphamide-induced immunosuppression effects in silkworm and their metabolic profiling analysis
    Li, Mei
    Xiao, Yang
    Yi, Yunhong
    Liu, Jiajie
    Wu, Gongqing
    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 2024, 27 (02)
  • [27] Transcriptome Analysis Provides Insights into the Mechanisms of Starch Biosynthesis in the Kernels of Three Chestnut Cultivars
    Wang, Wu
    Zhang, Shijie
    Chen, Yu
    Zhao, Yuqiang
    Shi, Fenghou
    Khalil-Ur-Rehman, Muhammad
    Bai, Xiaoqian
    Zhu, Cancan
    FORESTS, 2022, 13 (12):
  • [28] Tissue-specific transcriptome for Rheum tanguticum reveals candidate genes related to the anthraquinones biosynthesis
    Zhou, Tao
    Zhang, Tianyi
    Sun, Jiangyan
    Zhu, Honghong
    Zhang, Miao
    Wang, Xumei
    PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2021, 27 (11) : 2487 - 2501
  • [29] Tissue-specific transcriptome for Rheum tanguticum reveals candidate genes related to the anthraquinones biosynthesis
    Tao Zhou
    Tianyi Zhang
    Jiangyan Sun
    Honghong Zhu
    Miao Zhang
    Xumei Wang
    Physiology and Molecular Biology of Plants, 2021, 27 : 2487 - 2501
  • [30] Comparative transcriptome analysis reveals candidate genes for the biosynthesis of natural insecticide in Tanacetum cinerariifolium
    Khan, Sana
    Upadhyay, Swati
    Khan, Feroz
    Tandon, Sudeep
    Shukla, Rakesh Kumar
    Ghosh, Sumit
    Gupta, Vikrant
    Banerjee, Suchitra
    Rahman, Laiq Ur
    BMC GENOMICS, 2017, 18
12345