Transcriptome analysis of three cultivars of Poria cocos reveals genes related to the biosynthesis of polysaccharides

被引：15
|
作者：
Yang, Lan [1 ,2 ]
Tang, Juan [1 ,2 ]
Chen, Jin-Jun [1 ]
Peng, Ai-Yun [3 ]
Wang, Qi-Ming [1 ,2 ]
Rao, Li-Qun [1 ,2 ]
Yang, Hua [1 ,2 ]
Zhang, Xian-Wen [1 ,2 ]
Yang, Huan-Zhi [1 ,2 ]
Zhang, Chen [1 ,2 ]
Peng, Guo-Ping [1 ,2 ]
机构：
[1] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China
[2] Hunan Engn Lab Good Agr Practice & Comprehens Uti, Changsha 410128, Hunan, Peoples R China
[3] Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Guangdong, Peoples R China
来源：
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH | 2019年 / 21卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Poria cocos; polysaccharides; polysaccharides biosynthesis; transcriptome; qRT-PCR; BACTERIAL;
D O I：
10.1080/10286020.2018.1494159
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Poria cocos (P. cocos) polysaccharides (PCPs) are used to improve immunity and possess antitumor activities. We compared three cultivars of P. cocos (5.78, XJ 28 and JHYH) PCP contents. Then we determined that malZ, galA, SORD, gnl and bglX are key enzymes within the PCP biosynthetic pathway by using HiSeq2500 transcriptome and qRT-PCR validation. Our results provide more detailed information about the PCP biosynthesis pathway at the molecular level in P. cocos and establish the functions for the molecular breeding to produce polysaccharides in general for therapeutic use in Chinese medicinal plants. [GRAPHICS] .
引用
收藏
页码：462 / 475
页数：14
相关论文
共 50 条
  • [1] Transcriptome analysis reveals immune and metabolic regulation effects of Poria cocos polysaccharides on Bombyx mori larvae
    Liu, Jiajie
    Hong, Wanyu
    Li, Mei
    Xiao, Yang
    Yi, Yunhong
    Liu, Yi
    Wu, Gongqing
    FRONTIERS IN IMMUNOLOGY, 2022, 13
  • [2] De Novo Sequencing and Transcriptome Analysis of Wolfiporia cocos to Reveal Genes Related to Biosynthesis of Triterpenoids
    Shu, Shaohua
    Chen, Bei
    Zhou, Mengchun
    Zhao, Xinmei
    Xia, Haiyang
    Wang, Mo
    PLOS ONE, 2013, 8 (08):
  • [3] Isolation and structural analysis of polysaccharides from poria cocos mycelia
    Ding, Q
    Zhang, L
    Cheung, PCK
    ACTA POLYMERICA SINICA, 2000, (02) : 224 - 227
  • [4] Isolation and structural analysis of polysaccharides from the sclerotium of Poria cocos Wolf
    Zhang, LN
    Ding, Q
    Zhang, PY
    Feng, HQ
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 1997, 18 (06): : 990 - 993
  • [5] Transcriptome reveals insights into biosynthesis of ginseng polysaccharides
    Xiaoxue Fang
    Huaying Wang
    Xinteng Zhou
    Jing Zhang
    Hongxing Xiao
    BMC Plant Biology, 22
  • [6] Transcriptome reveals insights into biosynthesis of ginseng polysaccharides
    Fang, Xiaoxue
    Wang, Huaying
    Zhou, Xinteng
    Zhang, Jing
    Xiao, Hongxing
    BMC PLANT BIOLOGY, 2022, 22 (01)
  • [7] Transcriptome analysis reveals candidate genes related to steroid alkaloid biosynthesis in Fritillaria anhuiensis
    Shan, Tingyu
    Shi, Yuanyuan
    Xu, Jingyao
    Zhao, Liqiang
    Tao, Yijia
    Wu, Jiawen
    PHYSIOLOGIA PLANTARUM, 2022, 174 (04)
  • [8] Integrative analysis reveals the anti-obesity roles of Poria cocos polysaccharides through beneficial effects on gut microbiota
    Jiang, Shaofeng
    Liang, Cheng
    Wan, Xiaowei
    Lai, Keng Po
    Li, Rong
    Chen, Jian
    Liu, Jiaqi
    JOURNAL OF FUNCTIONAL FOODS, 2024, 119
  • [9] Transcriptome analysis of two Pogostemon cablin chemotypes reveals genes related to patchouli alcohol biosynthesis
    Yan, Wuping
    Ye, Zhouchen
    Cao, Shijia
    Yao, Guanglong
    Yu, Jing
    Yang, Dongmei
    Chen, Ping
    Zhang, Junfeng
    Wu, Yougen
    PEERJ, 2021, 9
  • [10] Transcriptome Analysis Reveals Candidate Genes Related to Anthocyanin Biosynthesis in Different Carrot Genotypes and Tissues
    Meng, Geng
    Clausen, Sabine K.
    Rasmussen, Soren K.
    PLANTS-BASEL, 2020, 9 (03):
12345