Purification of chlorogenic acid from Heijingang potatoes and evaluation of its binding properties to recombinant human serum albumin

被引：7

作者：

Zhang, Xueli ^{[1
,2
]}

Zeng, Lijun ^{[1
,2
]}

Sun, Tian ^{[1
,2
]}

Liu, Xixia ^{[1
,2
]}

Hou, Jianjun ^{[1
,2
]}

Ma, Qiuping ^{[1
,2
]}

Li, Yani ^{[1
,2
]}

Lu, Qi ^{[1
,2
]}

Chen, Sirui ^{[1
,2
]}

机构：

[1] Hubei Normal Univ, Hubei Key Lab Edible Wild Plants Conservat & Util, Cihu Rd, Huangshi 435002, Hubei, Peoples R China

[2] Hubei Normal Univ, Natl Demonstrat Ctr Expt Biol Educ, Huangshi 435002, Hubei, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2019年 / 1110卷

关键词：

Heijingang potato; Chlorogenic acid; Semipreparative liquid chromatography; Interaction; Recombinant human serum albumin; Molecular docking; MICROWAVE-ASSISTED EXTRACTION; EUPATORIUM-ADENOPHORUM; ANTIOXIDANT ACTIVITY; FLOWER BUDS; BY-PRODUCTS; SEPARATION; ANTHOCYANINS; EPICATECHIN;

D O I：

10.1016/j.jchromb.2019.02.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to purify the natural chlorogenic acid (CGA) monomer from Heijingang potatoes and investigate its interaction with recombinant human serum albumin (rHSA). The potato extract (PE) was purified using macroporous resins and solvent, and the CGA monomer was subsequently isolated using semipreparative liquid chromatography (SP-LC). The purity and structure of the CGA monomer was analyzed by high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). The interaction between the CGA monomer and rHSA was studied using fluorescence spectroscopy and molecular docking. HPLC analysis indicates that the CGA monomer had a retention time of 5.368 min and a purity of 97.9%, the presence of which was confirmed by NMR. The molecular docking and fluorescence spectroscopy demonstrate that CGA had a static quenching effect on rHSA with one binding site, and the range of K values was 7.14 x 10(3) to 1.56 x 10(4) M-1. This simple and efficient extract coupled with SP-LC has the potential for use in the extraction and purification of CGA in pilot or large-scale operations.

引用

页码：87 / 93

页数：7

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