Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season

被引：30

作者：

Rahman, Mahbubur ^{[1
]}

Vandennause, Mary F. ^{[2
]}

Kieke, Burney A. ^{[2
]}

Belongia, Edward A. ^{[2
]}

机构：

[1] Univ Texas Galveston, Med Branch, Ctr Interdisciplinary Res Womens Hlth, Galveston, TX 77555 USA

[2] Marshfield Clin Res Fdn & Matshfield Clin, Marshfield, WI 54449 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 62卷 / 02期

关键词：

influenza virus; rapid test; Binax NOW; direct fluorescent assay; sensitivity; specificity;

D O I：

10.1016/j.diagmicrobio.2007.10.012

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The Binax NOW Flu A and Flu B (Binax NOW), direct fluorescent assay (DFA), and viral culture were evaluated and compared with a composite of viral Culture or reverse transcription polymerase chain reaction (RT-PCR). Participants with medically attended acute respiratory illness were identified through active surveillance during the 2006 to 2007 season, and consenting individuals (n = 932) were tested for influenza by Culture and RT-PCR. Physicians ordered a rapid antigen test (Binax NOW [n = 73] or DFA [n = 70]) according to their clinical judgment. The Binax NOW detected 11 of 18 influenza infections (sensitivity, 61%; 95% confidence interval [CI], 36-83%)), whereas DFA detected 17 of 21 influenza infections (sensitivity 81%, 95% Cl, 58-95%). Compared with culture/RT-PCR, specificity of both Binax NOW and DFA was 100%. During the 2006 to 2007 Influenza season, DFA and Binax NOW demonstrated high specificity but failed to identify a substantial proportion Of Influenza infections. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：162 / 166

页数：5