Genome sequence of the model medicinal mushroom Ganoderma lucidum

被引：485

作者：

Chen, Shilin ^{[1
,2
]}

Xu, Jiang ^{[1
,2
]}

Liu, Chang ^{[1
,2
]}

Zhu, Yingjie ^{[1
,2
]}

Nelson, David R. ^{[3
]}

Zhou, Shiguo ^{[4
]}

Li, Chunfang ^{[1
,2
]}

Wang, Lizhi ^{[1
,2
]}

Guo, Xu ^{[1
,2
]}

Sun, Yongzhen ^{[1
,2
]}

Luo, Hongmei ^{[1
,2
]}

Li, Ying ^{[1
,2
]}

Song, Jingyuan ^{[1
,2
]}

Henrissat, Bernard ^{[5
]}

Levasseur, Anthony ^{[6
]}

Qian, Jun ^{[1
,2
]}

Li, Jianqin ^{[1
,2
]}

Luo, Xiang ^{[1
,2
]}

Shi, Linchun ^{[1
,2
]}

He, Liu ^{[1
,2
]}

Xiang, Li ^{[1
,2
]}

Xu, Xiaolan ^{[1
,2
]}

Niu, Yunyun ^{[1
,2
]}

Li, Qiushi ^{[1
,2
]}

Han, Mira V. ^{[7
]}

Yan, Haixia ^{[1
,2
]}

Zhang, Jin ^{[1
,2
]}

Chen, Haimei ^{[1
,2
]}

Lv, Aiping ^{[8
]}

Wang, Zhen ^{[1
,2
]}

Liu, Mingzhu ^{[1
,2
]}

Schwartz, David C. ^{[4
]}

Sun, Chao ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

[2] Peking Union Med Coll, Beijing 100193, Peoples R China

[3] Univ Tennessee, Ctr Hlth Sci, Dept Microbiol Immunol & Biochem, Memphis, TN 38163 USA

[4] Univ Wisconsin Madison, Lab Mol & Computat Genom, Dept Chem, Lab Genet,UW Biotechnol Ctr, Madison, WI 53706 USA

[5] Aix Marseille Univ, CNRS, UMR 7257, F-13288 Marseille 09, France

[6] Aix Marseille Univ, INRA, Biotechnol Champignons Filamenteux UMR1163, ESIL, F-13288 Marseille 09, France

[7] Natl Evolutionary Synth Ctr, Durham, NC 27705 USA

[8] China Acad Chinese Med Sci, Beijing 100700, Peoples R China

来源：

NATURE COMMUNICATIONS | 2012年 / 3卷

基金：

中国国家自然科学基金;

关键词：

LING ZHI-8 LZ-8; SECONDARY METABOLISM; IMMUNOMODULATORY PROTEIN; FAMILY; BIOSYNTHESIS; FUNGUS; CYTOCHROME-P450; ANNOTATION; DIVERSITY; MACHINERY;

D O I：

10.1038/ncomms1923

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ganoderma lucidum is a widely used medicinal macrofungus in traditional Chinese medicine that creates a diverse set of bioactive compounds. Here we report its 43.3-Mb genome, encoding 16,113 predicted genes, obtained using next-generation sequencing and optical mapping approaches. The sequence analysis reveals an impressive array of genes encoding cytochrome P450s (CYPs), transporters and regulatory proteins that cooperate in secondary metabolism. The genome also encodes one of the richest sets of wood degradation enzymes among all of the sequenced basidiomycetes. In all, 24 physical CYP gene clusters are identified. Moreover, 78 CYP genes are coexpressed with lanosterol synthase, and 16 of these show high similarity to fungal CYPs that specifically hydroxylate testosterone, suggesting their possible roles in triterpenoid biosynthesis. The elucidation of the G. lucidum genome makes this organism a potential model system for the study of secondary metabolic pathways and their regulation in medicinal fungi.

引用

页数：9

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