Lineage Tracing of Pf4-Cre Marks Hematopoietic Stem Cells and Their Progeny

被引：62

作者：

Calaminus, Simon D. J. ^{[2
]}

Guitart, Amelie ^{[1
]}

Sinclair, Amy ^{[1
]}

Schachtner, Hannah ^{[2
]}

Watson, Steve P. ^{[3
]}

Holyoake, Tessa L. ^{[1
]}

Kranc, Kamil R. ^{[1
]}

Machesky, Laura M. ^{[2
]}

机构：

[1] Univ Glasgow, Inst Canc Sci, Paul OGorman Leukaemia Res Ctr, Glasgow, Lanark, Scotland

[2] Univ Glasgow, Beatson Inst Canc Res, Glasgow, Lanark, Scotland

[3] Univ Birmingham, Ctr Cardiovasc Sci, Inst Biomed Res, Birmingham, W Midlands, England

来源：

PLOS ONE | 2012年 / 7卷 / 12期

基金：

英国生物技术与生命科学研究理事会;

关键词：

PLATELET-FUNCTION; TRANSGENIC MICE; CRE; MEGAKARYOPOIESIS; MEGAKARYOCYTE; ACTIVATION; EXPRESSION; REGULATOR; KNOCKOUTS; TISSUES;

D O I：

10.1371/journal.pone.0051361

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.

引用

页数：6

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