Immunohistochemical detection of nestin in pediatric brain tumors

被引:89
作者
Almqvist, PM
Mah, R
Lendahl, U
Jacobsson, B
Hendson, G
机构
[1] Karolinska Inst, Dept Clin Neurosci, Neurosurg Sect, Stockholm, Sweden
[2] Karolinska Hosp, Dept Cell & Mol Biol, S-17176 Stockholm, Sweden
[3] Karolinska Hosp, Dept Pathol & Cytol, S-17176 Stockholm, Sweden
[4] British Columbia Childrens Hosp, Dept Pathol, Vancouver, BC V6H 3V4, Canada
关键词
first trimester; telencephalon; nestin; vimentin; S-100; GFAP; immunohistochemistry; antigen retrieval; PNET; ependymoma; glioma;
D O I
10.1177/002215540205000203
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Nestin is an intermediate filament protein (IFP) expressed in undifferentiated cells during CNS development and in CNS tumors. Previous studies have arrived at different conclusions in terms of which types of CNS tumors express nestin. In this report we establish an immunohistochemical protocol using antigen retrieval, which significantly enhances staining with two polyclonal anti-nestin antisera, #130 and #4350. The staining pattern was identical for the two nestin antisera and very similar to that of vimentin, while glial fibrillary acidic protein (GFAP), immunoreactivity was absent from 9.5-week-old forebrain. The current study of 20 primary CNS tumors from pediatric patients included seven ependymomas, seven primitive neuroectodermal tumors (PNETs), five pilocytic astrocytomas, and one glioblastoma multiforme (GBM). All these tumors expressed nestin to various extents, in contrast to five brain metastases tested. Strong nestin immunoreactivity was found in malignant primary CNS tumors, whereas benign pilocytic astrocytomas showed low but consistent nestin expression. In all tumors nestin immunoreactivity was confined to the cytoplasm of tumor cells and was co-expressed with astrocyte markers vimentin, GFAP, and S-100. Vascular endothelial cells of all neoplasms also showed marked immunoreactivity for nestin and vimentin, whereas they were negative for GFAP and S-100. In conclusion, antiserum #4350 detected nestin in formalin-fixed, paraffin-embedded tissue sections by heat-induced antigen retrieval immunohistochemistry. Nestin was expressed in both highly malignant and low malignant gliomas, indicating the potential use of nestin as a diagnostic tumor marker in surgical pathology.
引用
收藏
页码:147 / 158
页数:12
相关论文
共 52 条
[31]   Molecular biology of neuronal intermediate filaments [J].
Liem, Ronald K. H. .
CURRENT OPINION IN CELL BIOLOGY, 1993, 5 (01) :12-16
[32]   REEXPRESSION OF THE INTERMEDIATE FILAMENT NESTIN IN REACTIVE ASTROCYTES [J].
LIN, RCS ;
MATESIC, DF ;
MARVIN, M ;
MCKAY, RDG ;
BRUSTLE, O .
NEUROBIOLOGY OF DISEASE, 1995, 2 (02) :79-85
[33]   An evolutionarily conserved region in the second intron of the human nestin gene directs gene expression to CNS progenitor cells and to early neural crest cells [J].
Lothian, C ;
Lendahl, U .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1997, 9 (03) :452-462
[34]   Identification of both general and region-specific embryonic CNS enhancer elements in the nestin promoter [J].
Lothian, C ;
Prakash, N ;
Lendahl, U ;
Wahlström, GM .
EXPERIMENTAL CELL RESEARCH, 1999, 248 (02) :509-519
[35]  
Marvin NJ, 1998, J CELL SCI, V111, P1951
[36]   Insights about brain tumors gained through immunohistochemistry and in situ hybridization of nuclear and phenotypic markers [J].
McKeever, PE .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1998, 46 (05) :585-594
[37]   MICROWAVE ANTIGEN RETRIEVAL FOR IMMUNOCYTOCHEMISTRY ON FORMALIN-FIXED, PARAFFIN-EMBEDDED POSTMORTEM CNS TISSUE [J].
MCQUAID, S ;
MCCONNELL, R ;
MCMAHON, J ;
HERRON, B .
JOURNAL OF PATHOLOGY, 1995, 176 (02) :207-216
[38]   EVALUATION OF ETHYLENEDIAMINETETRAACETIC ACID, TETRASODIUM SALT DIHYDRATE (EDTA)-TWEEN 20 TREATMENT VERSUS PROTEASE DIGESTION OF FORMALIN-FIXED TISSUE-SECTIONS FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS-ANTIGEN IN INFECTED OVINE LUNG [J].
MEEHAN, JT ;
CUTLIP, RC ;
LEHMKUHL, HD .
VETERINARY PATHOLOGY, 1989, 26 (04) :322-325
[39]  
Mokry J, 1998, Acta Medica (Hradec Kralove), V41, P73
[40]  
ORahilly R., 1994, The Embryonic Human Brain. An Atlas of Developmental Stages, V1