Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification

被引：39

作者：

Tian, Tian ^{[1
]}

Xiao, Heng ^{[1
]}

Zhang, Xiaolian ^{[2
]}

Peng, Shuang ^{[1
]}

Zhang, Xiaoe ^{[1
]}

Guo, Shan ^{[1
]}

Wang, Shaoru ^{[1
]}

Liu, Songmei ^{[3
]}

Zhou, Xin ^{[3
]}

Meyers, Craig ^{[4
]}

Zhou, Xiang ^{[1
,5
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Hubei, Peoples R China

[2] Wuhan Univ, Sch Med, Dept Immunol, State Key Lab Virol, Wuhan 430072, Hubei, Peoples R China

[3] Wuhan Univ, Zhongnan Hosp, Wuhan 430072, Hubei, Peoples R China

[4] Penn State Coll Med, Dept Microbiol & Immunol H107, Hershey, PA 17033 USA

[5] Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 01期

关键词：

MICRORNA DETECTION; FLUORESCENCE DETECTION; GRAPHENE OXIDE; NUCLEIC-ACIDS; CELL-FREE; CANCER; PROBES;

D O I：

10.1039/c2cc36728a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We reported an efficient strategy based on a strand displacement amplification for serum miRNA detection. In such a system, a multiplexed, sensitive and quick detection of miRNAs could be achieved through a combination of fluorescence labeled probes, a common primer and a polymerase. This could be potentially used in the clinical field to achieve early disease diagnosis and prognosis.

引用

页码：75 / 77

页数：3

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