Significant up-regulation of nestin protein in the neostriatum of MPTP-treated mice - Are the striatal astrocytes regionally activated after systemic MPTP administration?

被引:32
作者
Chen, LW [1 ]
Wei, LC
Qiu, Y
Liu, HL
Rao, ZR
Ju, G
Chan, YS
机构
[1] Fourth Mil Med Univ, Inst Neurosci, Xian 710032, Peoples R China
[2] Fourth Mil Med Univ, Xijing Hosp, Dept Radiotherapy, Xian 710032, Peoples R China
[3] Univ Hong Kong, Dept Physiol, Hong Kong, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
nestin protein; glia; caudate putamen; MPTP; Parkinsonism; mouse;
D O I
10.1016/S0006-8993(01)03253-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We are interested in the possible role of central glial cells in pathogenesis of Parkinson's disease of mammals. Parkinsonism model was induced by systemic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration, and the reactive glial cells were examined by immunocytochemical visualization of nestin protein in the brains and spinal cords of C57 mice. Abundant nestin-like immunoreactivity was predominately found in the caudate putamen of MPTP-treated mice and about 481-fold of nestin-ne immunoreactive cells increased compared with that of control animals, indicating that significant up-regulation of nestin protein occurred in these regions. Majority of nestin-like immunoreactive cells characterized with astrocytic profiles of multiple, radical and hypotrophic processes, and showed a distribution and dynamic patterns similar to that of glial fibrillary acid protein (GFAP)-immunoreactive cells in the caudate putamen. Double immuno fluorescence confirmed that 100% of nestin-like immunoreactive cells exhibited GFAP-immunoreactivity while nestin/ GFAP double-labeled cells constituted about 84% of total GFAP-immunoreactive cells in the caudate putamen, indicating these nestin-like immunoreactive cells belong to a reactive population of the astrocytes. On the other hand, no obvious changes of nestin- or GFAP-like immunoreactivities were detected in the globus pallidus, the substantia nigra and the ventral tegmental area after MPTP-treatment. The results have provided morphological evidence for the regional activation of astrocytic glial cells following systemic MPTP administration, suggesting that a large population of reactive striatal astrocytes might play an important role in initial pathogenesis or acute stage of Parkinson's disease in mammals. (C) 2002 Elsevier Science BY All rights reserved.
引用
收藏
页码:9 / 17
页数:9
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