Antioxidant activity of propolis extracts from Serbia: A polarographic approach

被引:24
作者
Potkonjak, Nebojsa I. [1 ]
Veselinovic, Dragan S. [2 ]
Novakovic, Miroslav M. [3 ]
Gorjanovic, Stanislava Z. [4 ]
Pezo, Lato L. [4 ]
Suznjevic, Desanka Z. [4 ]
机构
[1] Univ Belgrade, Vinca Inst Nucl Sci, Chem Dynam Lab, Belgrade 11001, Serbia
[2] Univ Belgrade, Fac Phys Chem, Belgrade 11001, Serbia
[3] Univ Belgrade, Inst Chem Technol & Met, Belgrade 11001, Serbia
[4] Inst Gen & Phys Chem, Belgrade 11158 118, Serbia
关键词
Propolis extract; Antioxidant activity; Hydrogen peroxide; Polarography; HYDROGEN-PEROXIDE; TOTAL PHENOLS; BRAZILIAN PROPOLIS; OXIDATION; CAPACITY; ORIGIN; ASSAY; POWER;
D O I
10.1016/j.fct.2012.07.029
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3614 / 3618
页数:5
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