Sex, flies and microarrays

被引：31
作者：
Churchill, GA
Oliver, B
机构：
[1] Jackson Lab, Bar Harbor, ME 04609 USA
[2] NIDDK, NIH, Cellular & Dev Biol Lab, Bethesda, MD USA
来源：
NATURE GENETICS | 2001年 / 29卷 / 04期
关键词：
D O I：
10.1038/ng1201-355
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
In a typical microarray study, experimental RNA samples are compared to a reference sample and expression ratios are clustered. A new study of the effects of sex, aging and genotype on gene expression in Drosophila melanogaster departs from this paradigm. Highly replicated comparisons generate measures of expression that are analyzed using classical analysis of variance (ANOVA) techniques, revealing substantial differences in gene expression between the two sexes as well as smaller effects of age and strain. Moreover, this study demonstrates that replication and experimental design are essential to detecting small but biologically important differences in expression patterns.
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页码：355 / 356
页数：2
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