Involvement of β-1,4-galactosyltransferase V in malignant transformation-associated changes in glycosylation

被引：37

作者：

Shirane, K

Sato, T

Segawa, K

Furukawa, K ^{[1
]}

机构：

[1] Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan

[2] Keio Univ, Sch Med, Dept Microbiol, Shinjuku Ku, Tokyo 1608582, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 265卷 / 02期

关键词：

D O I：

10.1006/bbrc.1999.1684

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In spite of marked changes in the glycosylation upon malignant transformation of cells, no biological significance of beta-1,4-galactosyltransferase (beta-1,4-GalT) activities has been elucidated. When beta-1,4-GalT activities toward 1 mM GlcNAc beta-S-pNP were determined using homogenates of NIH3T3 and its transformant, MTAg, MTAg contained 1.3 times higher activities. Northern blot analysis, however, revealed that the beta-1,4-GalT V gene expression increases by three times with a decrease in that of beta-1,4-GalT II by one-fifth and without significant changes in those of other beta-1,4-GalTs in MTAg. Analysis of beta-1,4-GalT V acceptor-specificity showed that the GlcNAc beta 1-->6Man group of the GlcNAc beta 1-->6(GlcNA beta 1 -->2)Man alpha 1- branch is galactosylated. These results indicate that changes in beta-1,4-GalT II and V activities are important for the altered glycosylation. (C) 1999 Academic Press.

引用

页码：434 / 438

页数：5

共 34 条

[31] The acceptor substrate specificity of human β4-galactosyltransferase V indicates its potential function in O-glycosylation [J].