Physical characterization and diel dynamics of different fractions of extracellular polysaccharides in an axenic culture of a benthic diatom

被引:89
作者
de Brouwer, JFC [1 ]
Wolfstein, K [1 ]
Stal, LJ [1 ]
机构
[1] Netherlands Inst Ecol, Ctr Estuarine & Coastal Ecol, NL-4400 AC Yerseke, Netherlands
关键词
axenic culture; daily cycle; diatom; extracellular polymeric substances; EPS; methods; microphytobenthos;
D O I
10.1017/S0967026201003419
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The excretion of extracellular polymeric substances (EPS) by an axenic culture of the benthic diatom Cylindrotheca closterium was investigated. Two sequential extraction steps proved to be sufficient to remove the bulk of the EPS present. Soluble EPS was recovered by a simple centrifugation step and represented a fraction that was not or was only loosely associated with diatom cells. For the extraction of bound EPS, different procedures were compared. The best results were obtained using distilled water as extraction solvent (1 h, 30 degreesC). The sugars that were recovered using this procedure were typically associated with aggregates of diatoms. In addition to the distinct differences in localization of the different types of EPS, their temporal dynamics differed in relation to the light-dark cycle. Soluble EPS were continuously released into the medium at a rate of 1.6 pg cell(-1) day(-1). In contrast, the production of bound EPS was highly light-dependent. In the dark, this bound EPS rapidly disappeared, probably as the result of its utilization by the diatoms.
引用
收藏
页码:37 / 44
页数:8
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