Establishment of a new teleost brain cell line (DLB-1) from the European sea bass and its use to study metal toxicology

被引:31
作者
Morcillo, Patricia [1 ]
Chaves-Pozo, Elena [2 ]
Meseguer, Jose [1 ]
Esteban, M. Angeles [1 ]
Cuesta, Alberto [1 ]
机构
[1] Univ Murcia, Fish Innate Immune Syst Grp, Dept Cellular Biol & Histol, Fac Biol, Campus Reg Excelencia Int Campus Mare Nostrum, E-30100 Murcia, Spain
[2] IEO, Ctr Oceanog Murcia, Carretera Azohia S-N, Murcia 30860, Spain
关键词
European sea bass; DLB-1 cell line; Metals; Apoptosis; Oxidative stress; NEURAL STEM-CELLS; OXIDATIVE STRESS; MOLECULAR CHARACTERIZATION; HEAVY-METALS; IN-VITRO; PROGENITOR-CELL; MARINE FISH; APOPTOSIS; CYTOTOXICITY; EXPRESSION;
D O I
10.1016/j.tiv.2016.10.005
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
In teleost fish, there are no commercial cell lines for the European sea.bass (Dicentrarchus labrax). Thus, we have established the sea bass brain (DLB-1) cell line, using a fish retrovirus for immortalization, which resemble epithelial cells and express glial cells markers. Exposure to metals [Cd, methylmercury (MeHg), Pb or As] produces cytotoxicity and induction of reactive oxygen species (ROS) production. Interestingly, cell cycle analysis of DLB-1 cells shows that exposure to metals alters it significantly. Moreover, all the metals induce apoptosis as indicated by sub-Go/G1 population and annexin V binding. Finally, exposure of DLB-1 cells to metals also produces significant alterations at gene expression level, which confirm the above functional results. This is the first study in which metal cytotoxicity has been evaluated in a fish brain cell line and results seem to support that DLB-1 cells are suitable for toxicological studies. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:91 / 100
页数:10
相关论文
共 73 条
[1]   Development and characterization of cell lines derived from rohu, Labeo rohita (Hamilton), and catla, Catla catla (Hamilton) [J].
Ahmed, V. P. Ishaq ;
Chandra, V. ;
Sudhakaran, R. ;
Kumar, S. Rajesh ;
Sarathi, M. ;
Babu, V. Sarath ;
Ramesh, B. ;
Hameed, A. S. Sahul .
JOURNAL OF FISH DISEASES, 2009, 32 (03) :211-218
[2]   EXPRESSION OF TWO METALLOTHIONEIN GENES IN DIFFERENT BRAIN REGIONS OF COMMON CARP [J].
Ali, K. Said ;
Ferencz, Agnes ;
Deer, Aranka K. ;
Nemcsok, J. ;
Hermesz, Edit .
ACTA BIOLOGICA HUNGARICA, 2009, 60 (02) :149-158
[3]  
ALI SF, 1992, NEUROTOXICOLOGY, V13, P637
[4]  
[Anonymous], 2005, EUROPEAN FOOD SAFETY, V236, P1, DOI DOI 10.2903/J.EFSA.2005.236
[5]  
Ariza ME, 1998, ENVIRON MOL MUTAGEN, V31, P352, DOI 10.1002/(SICI)1098-2280(1998)31:4<352::AID-EM8>3.3.CO
[6]  
2-G
[7]   Chronic dietary mercury exposure causes oxidative stress, brain lesions, and altered behaviour in Atlantic salmon (Salmo salar) parr [J].
Berntssen, MHG ;
Aatland, A ;
Handy, RD .
AQUATIC TOXICOLOGY, 2003, 65 (01) :55-72
[8]   Development of a cell line from the American eel brain expressing endothelial cell properties [J].
Bloch, Sophia R. ;
Vo, Nguyen T. K. ;
Walsh, Sarah K. ;
Chen, Cici ;
Lee, Lucy E. J. ;
Hodson, Peter V. ;
Bols, Niels C. .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2016, 52 (04) :395-409
[9]   Ecotoxicology and innate immunity in fish [J].
Bols, NC ;
Brubacher, JL ;
Ganassin, RC ;
Lee, LEJ .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2001, 25 (8-9) :853-873
[10]   TOXICITY DETERMINED INVITRO BY MORPHOLOGICAL ALTERATIONS AND NEUTRAL RED ABSORPTION [J].
BORENFREUND, E ;
PUERNER, JA .
TOXICOLOGY LETTERS, 1985, 24 (2-3) :119-124